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Literature summary extracted from

  • Rohdich, F.; Wungsintaweekul, J.; Luttgen, H.; Fischer, M.; Eisenreich, W.; Schuhr, C.A.; Fellermeier, M.; Schramek, N.; Zenk, M.H.; Bacher, A.
    Biosynthesis of terpenoids: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase from tomato (2000), Proc. Natl. Acad. Sci. USA, 97, 8251-8256.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.148 putative catalytic domain, residues 81-401 Solanum lycopersicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.148 Mg2+
-
Solanum lycopersicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.148 36400
-
calculated from cDNA sequence Solanum lycopersicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.148 ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol Solanum lycopersicum involved in terpenoid biosynthesis via deoxyxylulose phosphate pathway ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.148 Solanum lycopersicum P93841 tomato
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.148 recombinant enzyme Solanum lycopersicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.148 33
-
purified recombinant protein Solanum lycopersicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.148 ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
Solanum lycopersicum ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
?
2.7.1.148 ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol involved in terpenoid biosynthesis via deoxyxylulose phosphate pathway Solanum lycopersicum ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
-
?
2.7.1.148 additional information no conversion of isopentenyl phosphate into isopentenyl diphosphate Solanum lycopersicum ?
-
?