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Literature summary extracted from

  • Hummel, W.; Schuette, H.; Schmidt, E.; Wandrey, C.; Kula, M.R.
    Isolation of L-phenylalanine dehydrogenase from Rhodococcus sp. M4 and its application for the production of L-phenylalanine (1987), Appl. Microbiol. Biotechnol., 26, 409-416.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.4.1.20 synthesis continous production of L-Phe in an enzyme-membrane-reactor Rhodococcus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.20 L-Phe
-
Rhodococcus sp.
1.4.1.20 NAD+
-
Rhodococcus sp.
1.4.1.20 NADH
-
Rhodococcus sp.
1.4.1.20 phenylpyruvate
-
Rhodococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.20 0.13
-
NADH
-
Rhodococcus sp.
1.4.1.20 0.16
-
phenylpyruvate
-
Rhodococcus sp.
1.4.1.20 0.27
-
NAD+
-
Rhodococcus sp.
1.4.1.20 0.43
-
L-Met
-
Rhodococcus sp.
1.4.1.20 0.75
-
L-Phe
-
Rhodococcus sp.
1.4.1.20 2.1
-
2-oxo-4-methylthiobutanoate
-
Rhodococcus sp.
1.4.1.20 2.4
-
p-hydroxyphenylpyruvate
-
Rhodococcus sp.
1.4.1.20 3.1
-
L-Tyr
-
Rhodococcus sp.
1.4.1.20 7.7
-
Indolepyruvate
-
Rhodococcus sp.
1.4.1.20 11
-
L-Trp
-
Rhodococcus sp.
1.4.1.20 387
-
NH4+
-
Rhodococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.20 Rhodococcus sp.
-
M4
-
1.4.1.20 Rhodococcus sp. M4
-
M4
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.20
-
Rhodococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.20 2-oxo-4-methylthiobutanoate + NH3 + NADH 33% of the activity with L-Phe Rhodococcus sp. L-Met + NAD+ + H2O
-
?
1.4.1.20 4-hydroxyphenylpyruvate + NH3 + NADH 5% of the activity with L-Phe Rhodococcus sp. L-Tyr + NAD+ + H2O
-
?
1.4.1.20 4-hydroxyphenylpyruvate + NH3 + NADH 5% of the activity with L-Phe Rhodococcus sp. M4 L-Tyr + NAD+ + H2O
-
?
1.4.1.20 indolepyruvate + NH3 + NADH 3% of the activity with L-Phe Rhodococcus sp. ?
-
?
1.4.1.20 L-Met + H2O + NAD+ 4% of the activity with L-Phe Rhodococcus sp. 2-oxo-4-methylthiobutanoate + NH3 + NADH
-
?
1.4.1.20 L-Met + H2O + NAD+ 4% of the activity with L-Phe Rhodococcus sp. M4 2-oxo-4-methylthiobutanoate + NH3 + NADH
-
?
1.4.1.20 L-Phe + H2O + NAD+
-
Rhodococcus sp. phenylpyruvate + NH3 + NADH
-
r
1.4.1.20 L-Phe + H2O + NAD+
-
Rhodococcus sp. M4 phenylpyruvate + NH3 + NADH
-
r
1.4.1.20 L-Trp + H2O + NAD+ 2% of the activity with L-Phe Rhodococcus sp. indole-3-pyruvate + NH3 + NADH
-
?
1.4.1.20 L-Trp + H2O + NAD+ 2% of the activity with L-Phe Rhodococcus sp. M4 indole-3-pyruvate + NH3 + NADH
-
?
1.4.1.20 L-Tyr + H2O + NAD+
-
Rhodococcus sp. 4-hydroxyphenylpyruvate + NH3 + NADH
-
?
1.4.1.20 L-Tyr + H2O + NAD+
-
Rhodococcus sp. M4 4-hydroxyphenylpyruvate + NH3 + NADH
-
?

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.4.1.20 20 70 20°C: about 50% of maximal activity, 70°C: about 70% of maximal activity Rhodococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.20 9.3
-
reductive amination Rhodococcus sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.20 NAD+
-
Rhodococcus sp.
1.4.1.20 NADH
-
Rhodococcus sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.1.20 0.002
-
NADH
-
Rhodococcus sp.
1.4.1.20 0.07
-
phenylpyruvate
-
Rhodococcus sp.
1.4.1.20 1.27
-
NAD+
-
Rhodococcus sp.
1.4.1.20 7.34
-
phenylpyruvate
-
Rhodococcus sp.
1.4.1.20 17.85
-
Phe
-
Rhodococcus sp.