Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • De Boer, L.; Van Rijssel, M.; Euverink, G.J.; Dijkhuizen, L.
    Purification, characterization and regulation of a monomeric L-phenylalanine dehydrogenase from the facultative methylotroph Nocardia sp. 239 (1989), Arch. Microbiol., 153, 12-18.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.20 0.06
-
phenylpyruvate
-
Nocardia sp.
1.4.1.20 0.23
-
NAD+
-
Nocardia sp.
1.4.1.20 0.75
-
L-Phe
-
Nocardia sp.
1.4.1.20 96
-
NH3
-
Nocardia sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.20 42000
-
gel filtration Nocardia sp.

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.20 Nocardia sp.
-
239
-
1.4.1.20 Nocardia sp. 239
-
239
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.20 partial Nocardia sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.20 2-oxohexanoate + NH3 + NADH 240% of the activity with phenylpyruvate Nocardia sp. 2-aminohexanoate + H2O + NAD+
-
?
1.4.1.20 2-oxohexanoate + NH3 + NADH 240% of the activity with phenylpyruvate Nocardia sp. 239 2-aminohexanoate + H2O + NAD+
-
?
1.4.1.20 4-hydroxyphenylpyruvate + NH3 + NADH 28% of the activity with phenylpyruvate Nocardia sp. L-Tyr + NAD+ + H2O
-
?
1.4.1.20 4-hydroxyphenylpyruvate + NH3 + NADH 28% of the activity with phenylpyruvate Nocardia sp. 239 L-Tyr + NAD+ + H2O
-
?
1.4.1.20 indole-3-pyruvate + NH3 + NADH + H+ 54% of the activity with phenylpyruvate Nocardia sp. L-Trp + NAD+ + H2O
-
?
1.4.1.20 indole-3-pyruvate + NH3 + NADH + H+ 54% of the activity with phenylpyruvate Nocardia sp. 239 L-Trp + NAD+ + H2O
-
?
1.4.1.20 L-Leu + H2O + NAD+
-
Nocardia sp. 3-methyl-2-oxopentanoic acid + NH3 + NADH
-
?
1.4.1.20 L-Leu + H2O + NAD+
-
Nocardia sp. 239 3-methyl-2-oxopentanoic acid + NH3 + NADH
-
?
1.4.1.20 L-Phe + H2O + NAD+
-
Nocardia sp. phenylpyruvate + NH3 + NADH
-
r
1.4.1.20 L-Phe + H2O + NAD+
-
Nocardia sp. 239 phenylpyruvate + NH3 + NADH
-
r

Subunits

EC Number Subunits Comment Organism
1.4.1.20 monomer 1 * 42000, SDS-PAGE Nocardia sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.20 53
-
-
Nocardia sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.4.1.20 40 53 40°C: about 65% of maximal activity, 53°C: optimum, no activity at 55°C Nocardia sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.20 53
-
pH 6.0, 5 min, 50% loss of activity Nocardia sp.
1.4.1.20 53
-
phenylpyruvate destabilizes the activity, 1 M sorbitol or 1 M glycerol stabilize Nocardia sp.
1.4.1.20 53
-
pH 9.5-10, 2 h, 50% loss of activity Nocardia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.20 10
-
-
Nocardia sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.4.1.20 9.3 10.2 pH 9.3: about 40% of maximal activity, pH 10.2: about 35% of maximal activity Nocardia sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.20 additional information no activity with NADP+ Nocardia sp.
1.4.1.20 NAD+
-
Nocardia sp.
1.4.1.20 NADH
-
Nocardia sp.