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Literature summary extracted from
- Crystallization and properties of L-alanine dehydrogenase from Streptomyces phaeochromogenes (1983), Agric. Biol. Chem., 47, 2511-2519.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.1.1 | 1,10-phenanthroline | activation | Streptomyces phaeochromogenes |  |
| 1.4.1.1 | 2,2'-dipyridyl | activation | Streptomyces phaeochromogenes | |
| 1.4.1.1 | 2-mercaptoethanol | activation | Streptomyces phaeochromogenes | |
| 1.4.1.1 | diethyldithiocarbamate | activation | Streptomyces phaeochromogenes | |
| 1.4.1.1 | dithiothreitol | activation | Streptomyces phaeochromogenes | |
| 1.4.1.1 | EDTA | activation | Streptomyces phaeochromogenes | |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.4.1.1 | - |
Streptomyces phaeochromogenes |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.1.1 | Cu2+ | - |
Lysinibacillus sphaericus | |
| 1.4.1.1 | Cu2+ | - |
Streptomyces clavuligerus | |
| 1.4.1.1 | Cu2+ | - |
Streptomyces phaeochromogenes | |
| 1.4.1.1 | monoiodoacetate | - |
Streptomyces phaeochromogenes | |
| 1.4.1.1 | Pb2+ | - |
Lysinibacillus sphaericus | |
| 1.4.1.1 | Pb2+ | - |
Streptomyces phaeochromogenes | |
| 1.4.1.1 | PCMB | - |
Bacillus subtilis | |
| 1.4.1.1 | PCMB | - |
Lysinibacillus sphaericus | |
| 1.4.1.1 | PCMB | - |
Streptomyces phaeochromogenes | |
| 1.4.1.1 | Zn2+ | - |
Bacillus subtilis | |
| 1.4.1.1 | Zn2+ | - |
Streptomyces clavuligerus | |
| 1.4.1.1 | Zn2+ | - |
Streptomyces phaeochromogenes | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.4.1.1 | 0.036 | - |
NAD+ | pH 10.0 | Streptomyces phaeochromogenes | |
| 1.4.1.1 | 0.047 | - |
NADH | - |
Streptomyces phaeochromogenes | |
| 1.4.1.1 | 0.29 | - |
pyruvate | - |
Streptomyces phaeochromogenes | |
| 1.4.1.1 | 1.9 | - |
L-Ala | pH 8.0 | Streptomyces phaeochromogenes | |
| 1.4.1.1 | 3.6 | - |
L-Ala | pH 9.0 | Streptomyces phaeochromogenes | |
| 1.4.1.1 | 7.1 | - |
L-Ala | pH 10.0 | Streptomyces phaeochromogenes | |
| 1.4.1.1 | 61 | - |
NH4+ | - |
Streptomyces phaeochromogenes | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.1 | 39000 | - |
6 * 39000, SDS-PAGE | Streptomyces phaeochromogenes |
| 1.4.1.1 | 240000 | - |
gel filtration | Streptomyces phaeochromogenes |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.4.1.1 | Bacillus subtilis | - |
- |
- |
| 1.4.1.1 | Kitasatospora albolonga | - |
- |
- |
| 1.4.1.1 | Lysinibacillus sphaericus | - |
- |
- |
| 1.4.1.1 | Micromonospora melanosporea | - |
- |
- |
| 1.4.1.1 | Streptomyces albus | - |
- |
- |
| 1.4.1.1 | Streptomyces aureus | - |
- |
- |
| 1.4.1.1 | Streptomyces bobili | - |
- |
- |
| 1.4.1.1 | Streptomyces caespitosus | - |
- |
- |
| 1.4.1.1 | Streptomyces clavuligerus | - |
- |
- |
| 1.4.1.1 | Streptomyces coelicolor | - |
- |
- |
| 1.4.1.1 | Streptomyces flaveus | - |
- |
- |
| 1.4.1.1 | Streptomyces griseoluteus | - |
- |
- |
| 1.4.1.1 | Streptomyces griseus | - |
- |
- |
| 1.4.1.1 | Streptomyces hygroscopicus | - |
- |
- |
| 1.4.1.1 | Streptomyces lydicus | - |
- |
- |
| 1.4.1.1 | Streptomyces olivochromogenes | - |
- |
- |
| 1.4.1.1 | Streptomyces phaeochromogenes | - |
- |
- |
| 1.4.1.1 | Streptomyces roseochromogenus | - |
- |
- |
| 1.4.1.1 | Streptomyces roseus | - |
- |
- |
| 1.4.1.1 | Streptomyces ruber | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.4.1.1 | - |
Streptomyces phaeochromogenes |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.1 | 22.8 | - |
- |
Streptomyces phaeochromogenes |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.4.1.1 | 2-oxobutanoate + NH3 + NADH | - |
Lysinibacillus sphaericus | 2-aminobutanoate + H2O + NAD+ | - |
? | |
| 1.4.1.1 | 2-oxobutanoate + NH3 + NADH | 15.8% of the activity with pyruvate | Streptomyces phaeochromogenes | 2-aminobutanoate + H2O + NAD+ | - |
? | |
| 1.4.1.1 | 3-hydroxypyruvate + NH3 + NADH | - |
Lysinibacillus sphaericus | L-Ser + H2O + NAD+ | - |
? | |
| 1.4.1.1 | 3-hydroxypyruvate + NH3 + NADH | - |
Streptomyces clavuligerus | L-Ser + H2O + NAD+ | - |
? | |
| 1.4.1.1 | 3-hydroxypyruvate + NH3 + NADH | 32.3% of the activity with pyruvate | Streptomyces phaeochromogenes | L-Ser + H2O + NAD+ | - |
? | |
| 1.4.1.1 | glyoxylate + NH3 + NADH | - |
Lysinibacillus sphaericus | aminoacetate + NAD+ + H2O | - |
? | |
| 1.4.1.1 | glyoxylate + NH3 + NADH | - |
Streptomyces clavuligerus | aminoacetate + NAD+ + H2O | - |
? | |
| 1.4.1.1 | glyoxylate + NH3 + NADH | 13.3% of the activity with pyruvate | Streptomyces phaeochromogenes | aminoacetate + NAD+ + H2O | - |
? | |
| 1.4.1.1 | L-2-aminobutanoate + H2O + NAD+ | - |
Bacillus subtilis | 2-oxobutanoate + NH3 + NADH + H+ | - |
? | |
| 1.4.1.1 | L-2-aminobutanoate + H2O + NAD+ | - |
Lysinibacillus sphaericus | 2-oxobutanoate + NH3 + NADH + H+ | - |
? | |
| 1.4.1.1 | L-2-aminobutanoate + H2O + NAD+ | 1.6% of the activity with L-Ala | Streptomyces phaeochromogenes | 2-oxobutanoate + NH3 + NADH + H+ | - |
? | |
| 1.4.1.1 | L-Ala + H2O + NAD+ | - |
Bacillus subtilis | pyruvate + NH3 + NADH | - |
r | |
| 1.4.1.1 | L-Ala + H2O + NAD+ | - |
Lysinibacillus sphaericus | pyruvate + NH3 + NADH | - |
r | |
| 1.4.1.1 | L-Ala + H2O + NAD+ | - |
Streptomyces coelicolor | pyruvate + NH3 + NADH | - |
r | |
| 1.4.1.1 | L-Ala + H2O + NAD+ | - |
Streptomyces griseus | pyruvate + NH3 + NADH | - |
r | |
| 1.4.1.1 | L-Ala + H2O + NAD+ | - |
Streptomyces albus | pyruvate + NH3 + NADH | - |
r | |
| 1.4.1.1 | L-Ala + H2O + NAD+ | - |
Streptomyces olivochromogenes | pyruvate + NH3 + NADH | - |
r | |
| 1.4.1.1 | L-Ala + H2O + NAD+ | - |
Streptomyces phaeochromogenes | pyruvate + NH3 + NADH | - |
r | |
| 1.4.1.1 | L-Ala + H2O + NAD+ | - |
Streptomyces flaveus | pyruvate + NH3 + NADH | - |
r | |
| 1.4.1.1 | L-Ala + H2O + NAD+ | - |
Streptomyces bobili | pyruvate + NH3 + NADH | - |
r | |
| 1.4.1.1 | L-Ala + H2O + NAD+ | - |
Streptomyces roseochromogenus | pyruvate + NH3 + NADH | - |
r | |
| 1.4.1.1 | L-Ala + H2O + NAD+ | - |
Streptomyces ruber | pyruvate + NH3 + NADH | - |
r | |
| 1.4.1.1 | L-Ala + H2O + NAD+ | - |
Streptomyces aureus | pyruvate + NH3 + NADH | - |
r | |
| 1.4.1.1 | L-Ala + H2O + NAD+ | - |
Streptomyces lydicus | pyruvate + NH3 + NADH | - |
r | |
| 1.4.1.1 | L-Ala + H2O + NAD+ | - |
Streptomyces hygroscopicus | pyruvate + NH3 + NADH | - |
r | |
| 1.4.1.1 | L-Ala + H2O + NAD+ | - |
Streptomyces caespitosus | pyruvate + NH3 + NADH | - |
r | |
| 1.4.1.1 | L-Ala + H2O + NAD+ | - |
Streptomyces clavuligerus | pyruvate + NH3 + NADH | - |
r | |
| 1.4.1.1 | L-Ala + H2O + NAD+ | - |
Streptomyces griseoluteus | pyruvate + NH3 + NADH | - |
r | |
| 1.4.1.1 | L-Ala + H2O + NAD+ | - |
Kitasatospora albolonga | pyruvate + NH3 + NADH | - |
r | |
| 1.4.1.1 | L-Ala + H2O + NAD+ | - |
Micromonospora melanosporea | pyruvate + NH3 + NADH | - |
r | |
| 1.4.1.1 | L-Ala + H2O + NAD+ | - |
Streptomyces roseus | pyruvate + NH3 + NADH | - |
r | |
| 1.4.1.1 | L-Ser + H2O + NAD+ | - |
Bacillus subtilis | 3-hydroxypyruvate + NH3 + NADH | - |
? | |
| 1.4.1.1 | L-Ser + H2O + NAD+ | - |
Lysinibacillus sphaericus | 3-hydroxypyruvate + NH3 + NADH | - |
? | |
| 1.4.1.1 | L-Ser + H2O + NAD+ | 2.4% of the activity with L-Ala | Streptomyces phaeochromogenes | 3-hydroxypyruvate + NH3 + NADH | - |
? | |
| 1.4.1.1 | oxaloacetate + NH3 + NADH | - |
Lysinibacillus sphaericus | L-Asp + H2O + NAD+ | - |
? | |
| 1.4.1.1 | oxaloacetate + NH3 + NADH | 43.1% of the activity with pyruvate | Streptomyces phaeochromogenes | L-Asp + H2O + NAD+ | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.4.1.1 | hexamer | - |
Bacillus subtilis |
| 1.4.1.1 | hexamer | - |
Lysinibacillus sphaericus |
| 1.4.1.1 | hexamer | 6 * 39000, SDS-PAGE | Streptomyces phaeochromogenes |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.1 | 60 | - |
- |
Streptomyces phaeochromogenes |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.1 | 45 | - |
20 min, pH 7.0, stable | Streptomyces phaeochromogenes |
| 1.4.1.1 | 50 | - |
- |
Lysinibacillus sphaericus |
| 1.4.1.1 | 50 | - |
- |
Streptomyces coelicolor |
| 1.4.1.1 | 50 | - |
20 min, pH 7.0, 29% loss of activity | Streptomyces phaeochromogenes |
| 1.4.1.1 | 55 | - |
20 min, pH 7.0, 50% loss of activity | Streptomyces phaeochromogenes |
| 1.4.1.1 | 60 | - |
- |
Lysinibacillus sphaericus |
| 1.4.1.1 | 60 | - |
20 min, pH 7.0, 70% loss of activity | Streptomyces phaeochromogenes |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.1 | 8 | - |
reductive amination | Streptomyces phaeochromogenes |
| 1.4.1.1 | 10 | - |
oxidative deamination | Streptomyces phaeochromogenes |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.4.1.1 | 7 | 10 | 20°C, 3 h, stable | Streptomyces phaeochromogenes |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.1.1 | NAD+ | - |
Bacillus subtilis | |
| 1.4.1.1 | NAD+ | - |
Lysinibacillus sphaericus | |
| 1.4.1.1 | NAD+ | - |
Streptomyces coelicolor | |
| 1.4.1.1 | NAD+ | - |
Streptomyces griseus | |
| 1.4.1.1 | NAD+ | - |
Streptomyces albus | |
| 1.4.1.1 | NAD+ | - |
Streptomyces olivochromogenes | |
| 1.4.1.1 | NAD+ | - |
Streptomyces phaeochromogenes | |
| 1.4.1.1 | NAD+ | - |
Streptomyces flaveus | |
| 1.4.1.1 | NAD+ | - |
Streptomyces bobili | |
| 1.4.1.1 | NAD+ | - |
Streptomyces roseochromogenus | |
| 1.4.1.1 | NAD+ | - |
Streptomyces ruber | |
| 1.4.1.1 | NAD+ | - |
Streptomyces aureus | |
| 1.4.1.1 | NAD+ | - |
Streptomyces lydicus | |
| 1.4.1.1 | NAD+ | - |
Streptomyces hygroscopicus | |
| 1.4.1.1 | NAD+ | - |
Streptomyces caespitosus | |
| 1.4.1.1 | NAD+ | - |
Streptomyces clavuligerus | |
| 1.4.1.1 | NAD+ | - |
Streptomyces griseoluteus | |
| 1.4.1.1 | NAD+ | - |
Kitasatospora albolonga | |
| 1.4.1.1 | NAD+ | - |
Micromonospora melanosporea | |
| 1.4.1.1 | NAD+ | - |
Streptomyces roseus | |
| 1.4.1.1 | NADH | - |
Bacillus subtilis | |
| 1.4.1.1 | NADH | - |
Lysinibacillus sphaericus | |
| 1.4.1.1 | NADH | - |
Streptomyces coelicolor | |
| 1.4.1.1 | NADH | - |
Streptomyces griseus | |
| 1.4.1.1 | NADH | - |
Streptomyces albus | |
| 1.4.1.1 | NADH | - |
Streptomyces olivochromogenes | |
| 1.4.1.1 | NADH | - |
Streptomyces phaeochromogenes | |
| 1.4.1.1 | NADH | - |
Streptomyces flaveus | |
| 1.4.1.1 | NADH | - |
Streptomyces bobili | |
| 1.4.1.1 | NADH | - |
Streptomyces roseochromogenus | |
| 1.4.1.1 | NADH | - |
Streptomyces ruber | |
| 1.4.1.1 | NADH | - |
Streptomyces aureus | |
| 1.4.1.1 | NADH | - |
Streptomyces lydicus | |
| 1.4.1.1 | NADH | - |
Streptomyces hygroscopicus | |
| 1.4.1.1 | NADH | - |
Streptomyces caespitosus | |
| 1.4.1.1 | NADH | - |
Streptomyces clavuligerus | |
| 1.4.1.1 | NADH | - |
Streptomyces griseoluteus | |
| 1.4.1.1 | NADH | - |
Kitasatospora albolonga | |
| 1.4.1.1 | NADH | - |
Micromonospora melanosporea | |
| 1.4.1.1 | NADH | - |
Streptomyces roseus | |
| 1.4.1.1 | NADP+ | 0.6% of the activity with NAD+ | Streptomyces phaeochromogenes | |
| 1.4.1.1 | NADPH | 3.5% of the activity with NADH | Streptomyces phaeochromogenes | |
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