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Literature summary extracted from

  • Hester, K.L.; Luo, J.; Sokatch, J.R.
    Purification of Pseudomonas putida branched-chain keto acid dehydrogenase E1 component (2000), Methods Enzymol., 324, 129-138.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.4.4 thiamine diphosphate
-
 Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.4.4 expression of E1 component in Escherichia coli as a homotetramer, in Escherichia coli only one translational start site for E1beta is recognized and only the 39000 Da product is produced, in addition the N-terminal amino acid of the 39000 Da E1beta subunit is Met instead of Leu Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.4.4 167000
-
 E1 component, equilibrium sedimentation Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.4.4 additional information Pseudomonas putida enzyme complex is induced by growth on branched-chain amino acid or keto acid and repressed by growth in the presence of glucose or ammonium ion ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.4.4 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.4.4 E1 component, recombinant enzyme Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.4.4 additional information enzyme complex is induced by growth on branched-chain amino acid or keto acid and repressed by growth in the presence of glucose or ammonium ion Pseudomonas putida ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.4.4 CoA
-
 Pseudomonas putida
1.2.4.4 NAD+
-
 Pseudomonas putida