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Literature summary extracted from

  • Hein, S.; Steinbuechel, A.
    Cloning and characterization of the Alcaligenes eutrophus 2-oxoglutarate dehydrogenase complex (1996), FEMS Microbiol. Lett., 136, 231-238.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.4.2 gene odhL encodes the 2-oxoglutarate dehydrogenase component of the multienenzyme complex, expression in Escherichia coli in kgdA mutant of Pseudomonas putida JS347 Cupriavidus necator
2.3.1.61 cloning of the whole 2-oxoglutarase dehydrogenase complex structural genes, heterologous expression in Escherichia coli XL1-Blue and in the kgdA mutant Pseudomonas putida JS 347 Cupriavidus necator

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.61 mitochondrion
-
 Rattus norvegicus 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.61 succinyl-CoA + dihydrolipoamide Cupriavidus necator
-
 CoA + S-succinyldihydrolipoamide
-
 ?
2.3.1.61 succinyl-CoA + dihydrolipoamide Azotobacter vinelandii
-
 CoA + S-succinyldihydrolipoamide
-
 ?
2.3.1.61 succinyl-CoA + dihydrolipoamide Bacillus subtilis
-
 CoA + S-succinyldihydrolipoamide
-
 ?
2.3.1.61 succinyl-CoA + dihydrolipoamide Rattus norvegicus
-
 CoA + S-succinyldihydrolipoamide
-
 ?
2.3.1.61 succinyl-CoA + dihydrolipoamide Saccharomyces cerevisiae
-
 CoA + S-succinyldihydrolipoamide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.4.2 Cupriavidus necator
-
-
-
2.3.1.61 Azotobacter vinelandii P20708
-
-
2.3.1.61 Bacillus subtilis P16263
-
-
2.3.1.61 Cupriavidus necator
-
 H16, wild-type, ATCC 17699
-
2.3.1.61 Rattus norvegicus Q01205
-
-
2.3.1.61 Saccharomyces cerevisiae P19262 yeast
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.2.4.2 2-oxoglutarate + [dihydrolipoyllysine-residue succinyltransferase] lipoyllysine = [dihydrolipoyllysine-residue succinyltransferase] S-succinyldihydrolipoyllysine + CO2 the enzyme complex catalyzes the reaction : 2-oxoglutarate + CoA + NAD+--> succinyl-CoA + CO2 + NADH, the following partial reactions are catalyzed: 1. HOOC(CH2)2COCOOH + (thiamine diphosphate)-E1--> (HOOC(CH2)2 CHOH-thiamine-diphosphate)-E1 + CO2, 2. (HOOC(CH2)2CH OH-thiamine-diphosphate)-E1 + (LipS2)-E2--> (HOOC(CH)2 CO-(SLipSH))-E2 + (thiamine-diphosphate)-E1, 3. (HOOC(CH2)2CO-(SLipSH))-E2 + HSCoA--> (Lip(SH)2)-E2 + HOOC(CH2)2CO-SCoA, 4. (Lip(SH)2)-E2 + E3-FAD--> (LipS2)-E2 + reduced E3-FAD, 5. reduced E3-FAD + NAD+--> E3-FAD + NADH Cupriavidus necator
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.4.2 2-oxoglutarate + lipoamide
-
 Cupriavidus necator S-succinyldihydrolipoamide + CO2
-
 ?
2.3.1.61 succinyl-CoA + dihydrolipoamide
-
 Cupriavidus necator CoA + S-succinyldihydrolipoamide
-
 ?
2.3.1.61 succinyl-CoA + dihydrolipoamide
-
 Azotobacter vinelandii CoA + S-succinyldihydrolipoamide
-
 ?
2.3.1.61 succinyl-CoA + dihydrolipoamide
-
 Bacillus subtilis CoA + S-succinyldihydrolipoamide
-
 ?
2.3.1.61 succinyl-CoA + dihydrolipoamide
-
 Rattus norvegicus CoA + S-succinyldihydrolipoamide
-
 ?
2.3.1.61 succinyl-CoA + dihydrolipoamide
-
 Saccharomyces cerevisiae CoA + S-succinyldihydrolipoamide
-
 ?