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Literature summary extracted from
- Pyruvate dehydrogenase from Azotobacter vinelandii: properties of the N-terminally truncated enzyme (1999), Eur. J. Biochem., 265, 1098-1107.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.2.4.1 | expression of wild-type pyruvate dehydrogenase and N-terminal deletion mutant E1pDelta48 in Escherichia coli | Azotobacter vinelandii |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.2.4.1 | pyruvate | - |
Azotobacter vinelandii |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.2.4.1 | 0.024 | - |
lipoyl domain | acyl group transfer reaction, recombinant pyruvate dehydrogenase with N-terminal deletion | Azotobacter vinelandii | |
| 1.2.4.1 | 0.027 | - |
lipoyl domain | acyl group transfer reaction, recombinant wild-type pyruvate dehydrogenase | Azotobacter vinelandii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.2.4.1 | Azotobacter vinelandii | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.2.4.1 | pyruvate + lipoyl domain | - |
Azotobacter vinelandii | acetylated lipoyl domain + CO2 | - |
r | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.2.4.1 | 0.9 | - |
lipoyl domain | acyl group transfer reaction, recombinant wild-type pyruvate dehydrogenase | Azotobacter vinelandii | |
| 1.2.4.1 | 1.1 | - |
lipoyl domain | acyl group transfer reaction, recombinant pyruvate dehydrogenase with N-terminal deletion | Azotobacter vinelandii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.2.4.1 | 7 | - |
wild-type pyruvate dehydrogenase and N-terminal deletion mutant | Azotobacter vinelandii |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.2.4.1 | 31 | - |
pyruvate | recombinant wild-type pyruvate dehydrogenase | Azotobacter vinelandii | |
| 1.2.4.1 | 37 | - |
pyruvate | recombinant pyruvate dehydrogenase with N-terminal deletion | Azotobacter vinelandii | |
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Release 2023.1 (January 2023)
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