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Literature summary extracted from

  • Jeng, J.; Kallarakal, A.T.; Kim, S.F.; Popov, K.M.; Song, B.J.
    Pyruvate dehydrogenase E1alpha isoform in rat testis: cDNA cloning, characterization, and biochemical comparison of the recombinant testis and liver enzymes (1998), Comp. Biochem. Physiol. B, 120, 205-216.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.4.1 co-expression of alpha subunit with human liver beta subunit in Escherichia coli Homo sapiens
1.2.4.1 co-expression of alpha subunit with human liver beta subunit in Escherichia coli Rattus norvegicus
1.2.4.1 expression of alpha and beta subunits in Escherichia coli Homo sapiens
1.2.4.1 expression of alpha and beta subunits in Escherichia coli Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.4.1 0.0009
-
 thiamine diphosphate recombinant enzyme Rattus norvegicus
1.2.4.1 0.0015
-
 thiamine diphosphate recombinant enzyme Homo sapiens
1.2.4.1 0.0204
-
 pyruvate recombinant enzyme Rattus norvegicus
1.2.4.1 0.026
-
 pyruvate recombinant enzyme Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.2.4.1 Homo sapiens
-
-
-
1.2.4.1 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.4.1 testis
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.4.1 3.26
-
 recombinant enzyme from testis Rattus norvegicus
1.2.4.1 8.13
-
 recombinant enzyme from liver Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.4.1 acetaldehyde + benzaldehyde
-
 Homo sapiens (R)-phenylacetylcarbinol
-
 ?
1.2.4.1 acetaldehyde + benzaldehyde
-
 Rattus norvegicus (R)-phenylacetylcarbinol
-
 ?
1.2.4.1 pyruvate + CoA + NAD+
-
 Rattus norvegicus acetyl-CoA + CO2 + NADH
-
 ?