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Literature summary extracted from

  • Hamada, M.; Hiraoka, T.; Koike, K.; Ogasahara, K.; Kanzaki, T.; Koike, M.
    Properties and subunit structure of pig heart pyruvate dehydrogenase (1976), J. Biochem., 79, 1273-1285.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.4.1 2,2'-Dithiopyridine 1.1 mM, 30% inhibition Sus scrofa
1.2.4.1 5,5'-dithiobis(2-nitrobenzoate) 1.1 mM, 35% inhibition Sus scrofa
1.2.4.1 AgNO3 18 mol per mol enzyme, complete inhibition Sus scrofa
1.2.4.1 Aluminium sulfate 1.1 mM, 35% inhibition Sus scrofa
1.2.4.1 Ba2+ 0.11 mM, 10% inhibition Sus scrofa
1.2.4.1 Cd2+ 0.032 mM, complete inhibition Sus scrofa
1.2.4.1 citrate noncompetitive inhibition Sus scrofa
1.2.4.1 Cu2+ 0.36 mM, complete inhibition Sus scrofa
1.2.4.1 EDTA 0.036 mM, 40% inhibition, 0.36 mM, complete inhibition, reversed by addition of excess Mg2+ and Ca2+ Sus scrofa
1.2.4.1 Mn2+ 0.72 mM Sus scrofa
1.2.4.1 N-ethylmaleimide 1.1 mM, 50% inhibition Sus scrofa
1.2.4.1 p-chloromercuribenzenesulfonate 8 mol per mol enzyme, complete inhibition Sus scrofa
1.2.4.1 p-chloromercuribenzoate 8 mol per mol enzyme, complete inhibition Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.4.1 0.065
-
 thiamine diphosphate in the presence of Ca2+ and Mg2+ Sus scrofa
1.2.4.1 0.14
-
 pyruvate in the presence of Ca2+ and Mg2+ Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.4.1 mitochondrion
-
 Sus scrofa 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.4.1 Ca2+ can replace Mg2+ Sus scrofa
1.2.4.1 Ca2+ 0.36 mM, 5fold activation Sus scrofa
1.2.4.1 Mg2+ 0.36 mM, 5fold activation Sus scrofa
1.2.4.1 Mn2+ can replace Mg2+ Sus scrofa
1.2.4.1 Mn2+ 0.11 mM, 2fold activation Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.4.1 150000
-
 calculation from sedimentation and diffusion data, low speed sedimentation equilibrium centrifugation, meniscus depletion method Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.4.1 pyruvate + lipoamide Sus scrofa in overall reaction of pyruvate dehydrogenase complex, pyruvate can be replaced by 2-ketobutyrate S-acetyldihydrolipoamide + CO2
-
 ?
1.2.4.1 pyruvate + lipoamide Sus scrofa low reactivity with 2-ketovalerate S-acetyldihydrolipoamide + CO2
-
 ?
1.2.4.1 pyruvate + lipoamide Sus scrofa low reactivity with 2-ketoisovalerate S-acetyldihydrolipoamide + CO2
-
 ?
1.2.4.1 pyruvate + lipoamide Sus scrofa low reactivity with 2-ketoisocaproate S-acetyldihydrolipoamide + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.4.1 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.4.1 pyruvate dehydrogenase Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.4.1 heart
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.4.1 11
-
-
 Sus scrofa

Storage Stability

EC Number Storage Stability Organism
1.2.4.1 -18°C, 50 mM potassium phosphate, pH 7.0, 0.5 mM EDTA, several months, no loss of activity Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.4.1 acetaldehyde + benzaldehyde
-
 Sus scrofa (R)-phenylacetylcarbinol
-
 ?
1.2.4.1 alpha-ketobutyrate + lipoamide
-
 Sus scrofa S-propionyldihydrolipoamide + CO2
-
 ?
1.2.4.1 pyruvate + lipoamide in overall reaction of pyruvate dehydrogenase complex, pyruvate can be replaced by 2-ketobutyrate Sus scrofa S-acetyldihydrolipoamide + CO2
-
 ?
1.2.4.1 pyruvate + lipoamide low reactivity with 2-ketovalerate Sus scrofa S-acetyldihydrolipoamide + CO2
-
 ?
1.2.4.1 pyruvate + lipoamide low reactivity with 2-ketoisovalerate Sus scrofa S-acetyldihydrolipoamide + CO2
-
 ?
1.2.4.1 pyruvate + lipoamide low reactivity with 2-ketoisocaproate Sus scrofa S-acetyldihydrolipoamide + CO2
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.4.1 40
-
-
 Sus scrofa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.4.1 51
-
 1 min, complete inactivation Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.4.1 6.5 7.5 with Ca2+ as divalent cation Sus scrofa
1.2.4.1 6.5 7 with Mg2+ as divalent cation Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.4.1 thiamine diphosphate
-
 Sus scrofa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.2.4.1 8.6
-
 citrate
-
 Sus scrofa