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Literature summary extracted from

  • Treede, J.; Heise, K.P.
    Purification of the chloroplast pyruvate dehydrogenase complex from spinach and maize mesophyll (1986), Z. Naturforsch. C, 41, 1011-1017.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.2.1.104 very sensitive to freezing and thawing Spinacia oleracea
1.2.1.104 very sensitive to freezing and thawing Pisum sativum
1.2.1.104 very sensitive to freezing and thawing Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.104 chloroplast
-
 Spinacia oleracea 9507
-
1.2.1.104 chloroplast
-
 Pisum sativum 9507
-
1.2.1.104 chloroplast
-
 Zea mays 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.104 pyruvate + lipoamide Spinacia oleracea in overall reaction of pyruvate dehydrogenase complex, pyruvate can be replaced by hydroxypyruvate S-acetyldihydrolipoamide + CO2
-
 ?
1.2.1.104 pyruvate + lipoamide Pisum sativum in overall reaction of pyruvate dehydrogenase complex, pyruvate can be replaced by hydroxypyruvate S-acetyldihydrolipoamide + CO2
-
 ?
1.2.1.104 pyruvate + lipoamide Zea mays in overall reaction of pyruvate dehydrogenase complex, pyruvate can be replaced by hydroxypyruvate S-acetyldihydrolipoamide + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.104 Pisum sativum
-
-
-
1.2.1.104 Spinacia oleracea
-
 spinach
-
1.2.1.104 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.104 pyruvate dehydrogenase complex composed of EC 1.2.4.1, EC 2.3.1.12, EC 1.8.1.4 Spinacia oleracea
1.2.1.104 pyruvate dehydrogenase complex composed of EC 1.2.4.1, EC 2.3.1.12, EC 1.8.1.4 Zea mays

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.104 0.00085
-
 enzyme from chloroplast Spinacia oleracea
1.2.1.104 0.00265
-
 enzyme from chloroplast Zea mays
1.2.1.104 0.00485
-
 enzyme from mitochondrion Pisum sativum

Storage Stability

EC Number Storage Stability Organism
1.2.1.104 -20°C, 50 mM glycyl-glycine buffer, pH 8.0, 2 mM MgCl2, 2 mM DTT, 1-2 weeks Spinacia oleracea
1.2.1.104 -20°C, 50 mM glycyl-glycine buffer, pH 8.0, 2 mM MgCl2, 2 mM DTT, 1-2 weeks Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.104 pyruvate + lipoamide in overall reaction of pyruvate dehydrogenase complex, pyruvate can be replaced by hydroxypyruvate Spinacia oleracea S-acetyldihydrolipoamide + CO2
-
 ?
1.2.1.104 pyruvate + lipoamide in overall reaction of pyruvate dehydrogenase complex, pyruvate can be replaced by hydroxypyruvate Pisum sativum S-acetyldihydrolipoamide + CO2
-
 ?
1.2.1.104 pyruvate + lipoamide in overall reaction of pyruvate dehydrogenase complex, pyruvate can be replaced by hydroxypyruvate Zea mays S-acetyldihydrolipoamide + CO2
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.104 additional information
-
-
 Pisum sativum
1.2.1.104 additional information
-
 value for pyruvate dehydrogenase complex: 8.2, chloroplast enzyme Spinacia oleracea
1.2.1.104 additional information
-
 value for pyruvate dehydrogenase complex: 8.2, chloroplast enzyme Zea mays