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Literature summary extracted from

  • Miernyk, J.A.; Randall, D.D.
    Some kinetic and regulatory properties of the pea mitochondrial pyruvate dehydrogenase complex (1987), Plant Physiol., 83, 306-310.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.104 glyoxylate competitive vs. pyruvate Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.104 0.00008
-
 thiamine diphosphate whole enzyme complex Pisum sativum
1.2.1.104 0.36
-
 Mg2+ whole enzyme complex Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.104 mitochondrion
-
 Pisum sativum 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.104 Ca2+ can replace Mg2+ Pisum sativum
1.2.1.104 Mg2+ absolute requirement Pisum sativum
1.2.1.104 Mn2+ can replace Mg2+ Pisum sativum
1.2.1.104 additional information no activation by other divalent cations, except Mg2+, Mn2+, Ca2+, Ni2+ Pisum sativum
1.2.1.104 Ni2+ can replace Mg2+ Pisum sativum

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.104 Pisum sativum
-
 pea
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.104 pyruvate dehydrogenase complex composed of EC 1.2.4.1, EC 2.3.1.12, EC 1.8.1.4 Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.104 leaf
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.104 0.0513
-
 assay with whole enzyme complex Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.104 acetaldehyde + benzaldehyde
-
 Pisum sativum (R)-phenylacetylcarbinol
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.104 additional information
-
 values for pyruvate dehydrogenase complex: 35-50°C Pisum sativum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.1.104 15 50 value for pyruvate dehydrogenase complex Pisum sativum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.104 50
-
 irreversible inactivation Pisum sativum