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Literature summary extracted from

  • Eckfeldt, J.; Mope, L.; Takio, K.; Yonetani, T.
    Horse liver aldehyde dehydrogenase. Purification and characterization of two isozymes (1976), J. Biol. Chem., 251, 236-240.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.5 Chloral hydrate 50% inhibition of isoenzyme F1 by 0.25 mM, 50% inhibition of isoenzyme F2 by 0.4 mM Equus caballus
1.2.1.5 Disulfiram
-
 Equus caballus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.5 0.0001
-
 pentanal isoenzyme F1 Equus caballus
1.2.1.5 0.0002
-
 acetaldehyde isoenzyme F2 Equus caballus
1.2.1.5 0.0003
-
 phenylacetaldehyde isoenzyme F2 Equus caballus
1.2.1.5 0.0005
-
 isopentanal isoenzyme F1 Equus caballus
1.2.1.5 0.003
-
 NAD+ isoenzyme F1 Equus caballus
1.2.1.5 0.003
-
 NAD+ reaction with acetaldehyde Equus caballus
1.2.1.5 0.004
-
 phenylacetaldehyde isoenzyme F1 Equus caballus
1.2.1.5 0.005
-
 propanal isoenzyme F1 Equus caballus
1.2.1.5 0.01
-
 NADP+ isoenzyme F2 Equus caballus
1.2.1.5 0.01
-
 Chloroacetaldehyde isoenzyme F2 Equus caballus
1.2.1.5 0.01
-
 NADP+ reaction with acetaldehyde Equus caballus
1.2.1.5 0.03
-
 NAD+ isoenzyme F2 Equus caballus
1.2.1.5 0.03
-
 Chloroacetaldehyde isoenzyme F1 Equus caballus
1.2.1.5 0.03
-
 NAD+ reaction with acetaldehyde Equus caballus
1.2.1.5 0.05
-
 glycolaldehyde isoenzyme F2 Equus caballus
1.2.1.5 0.07
-
 acetaldehyde isoenzyme F1 Equus caballus
1.2.1.5 0.13
-
 glycolaldehyde isoenzyme F1 Equus caballus
1.2.1.5 0.27
-
 formaldehyde isoenzyme F2 Equus caballus
1.2.1.5 0.7
-
 NADP+ isoenzyme F1 Equus caballus
1.2.1.5 0.7
-
 NADP+ reaction with acetaldehyde Equus caballus
1.2.1.5 0.94
-
 formaldehyde isoenzyme F1 Equus caballus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.5 52000
-
 4 * 52000, isoenzyme F1, SDS-PAGE Equus caballus
1.2.1.5 53000
-
 4 * 53000, isoenzyme F2, SDS-PAGE Equus caballus
1.2.1.5 230000
-
 isoenzyme F1, non-denaturing PAGE Equus caballus
1.2.1.5 240000
-
 isoenzyme F2, non-denaturing PAGE Equus caballus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.5 additional information Equus caballus isoenzyme f1 may be the primary enzyme for oxidizing the acetaldehyde produced during ethanol oxidation in vivo ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.5 Equus caballus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.5 isoenzyme F1 and F2 Equus caballus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.5 liver
-
 Equus caballus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.5 0.35
-
 isoenzyme F1 Equus caballus
1.2.1.5 0.8
-
-
 Equus caballus
1.2.1.5 1
-
 isoenzyme F2 Equus caballus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.5 acetaldehyde + NAD+ + H2O
-
 Equus caballus acetate + NADH + H+
-
 ?
1.2.1.5 acetaldehyde + NADP+ + H2O
-
 Equus caballus acetate + NADPH + H+
-
 ?
1.2.1.5 benzaldehyde + NAD+ + H2O
-
 Equus caballus benzoate + NADH + H+
-
 ?
1.2.1.5 chloroacetaldehyde + NAD+ + H2O
-
 Equus caballus chloroacetate + NADH + H+
-
 ?
1.2.1.5 cinnamaldehyde + NAD+ + H2O
-
 Equus caballus cinnamate + NADH + H+
-
 ?
1.2.1.5 formaldehyde + NAD+ + H2O
-
 Equus caballus formate + NADH + H+
-
 ?
1.2.1.5 glycolaldehyde + NADP+ + H2O
-
 Equus caballus glycolate + NADPH + H+
-
 ?
1.2.1.5 isopentanal + NAD+ + H2O
-
 Equus caballus isopentanoate + NADH + H+
-
 ?
1.2.1.5 additional information isoenzyme f1 may be the primary enzyme for oxidizing the acetaldehyde produced during ethanol oxidation in vivo Equus caballus ?
-
 ?
1.2.1.5 n-pentanal + NAD+ + H2O
-
 Equus caballus n-pentanoate + NADH + H+
-
 ?
1.2.1.5 phenylacetaldehyde + NAD+ + H2O
-
 Equus caballus phenylacetate + NADH + H+
-
 ?
1.2.1.5 propanal + NAD+ + H2O
-
 Equus caballus propionate + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.5 tetramer 4 * 52000, isoenzyme F1, SDS-PAGE Equus caballus
1.2.1.5 tetramer 4 * 53000, isoenzyme F2, SDS-PAGE Equus caballus

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.5 NADP+ relative but not absolute specificity for NAD+ over NADP+ Equus caballus