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Literature summary extracted from

  • Moore, C.; Lei, B.; Tu, S.C.
    Relationship between the conserved alpha subunit arginine 107 and effects of phosphate on the activity and stability of Vibrio harveyi luciferase (1999), Arch. Biochem. Biophys., 370, 45-50.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.3 alphaR107A lower affinity for FMNH Vibrio harveyi
1.14.14.3 alphaR107E lower affinity for FMNH Vibrio harveyi
1.14.14.3 alphaR107S lower affinity for FMNH Vibrio harveyi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.3 0.0003
-
 decanal wild type, 50 mM phosphate Vibrio harveyi
1.14.14.3 0.0008
-
 FMNH wild type, 10 mM phosphate Vibrio harveyi
1.14.14.3 0.0018
-
 FMNH wild type, 300 mM phosphate Vibrio harveyi
1.14.14.3 0.0021
-
 decanal alphaR107S, 50 mM phosphate Vibrio harveyi
1.14.14.3 0.0022
-
 decanal alphaR107E, 50 mM phosphate Vibrio harveyi
1.14.14.3 0.0031
-
 decanal alphaR107A, 50 mM phosphate Vibrio harveyi
1.14.14.3 0.0038
-
 FMNH alphaR107S, 10 mM phosphate Vibrio harveyi
1.14.14.3 0.0049
-
 FMNH alphaR107S, 300 mM phosphate Vibrio harveyi
1.14.14.3 0.0081
-
 FMNH alphaR107A, 10 mM phosphate Vibrio harveyi
1.14.14.3 0.0095
-
 FMNH alphaR107A, 300 mM phosphate Vibrio harveyi
1.14.14.3 0.0114
-
 FMNH alphaR107E, 300 mM phosphate Vibrio harveyi
1.14.14.3 0.0234
-
 FMNH alphaR107E, 10 mM phosphate Vibrio harveyi

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.3 Vibrio harveyi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.3
-
 Vibrio harveyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.3 decanal + FMNH + O2
-
 Vibrio harveyi decanoic acid + FMN + H2O + light
-
 ir
1.14.14.3 dodecanal + FMNH + O2
-
 Vibrio harveyi dodecanoic acid + FMN + H2O + light
-
 ir
1.14.14.3 octanal + FMNH + O2
-
 Vibrio harveyi octanoic acid + FMN + H2O + light
-
 ir