Literature summary extracted from

Wienhausen, G.K.; Kricka, L.J.; Hinkley, J.E.; DeLuca, M.
Properties of bacterial luciferase/NADH:FMN oxidoreductase and firefly luciferase immobilized onto sepharose (1982), Appl. Biochem. Biotechnol., 7, 463-473.

General Stability

EC Number	General Stability	Organism
1.14.14.3	repeated freezing/thawing causes inactivation of immobilized enzyme	Vibrio harveyi

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.14.14.3	decanal + FMNH + O2	Vibrio harveyi	-	decanoic acid + FMN + H2O + light	-	ir

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.14.3	Vibrio harveyi	-	-	-

Storage Stability

EC Number	Storage Stability	Organism
1.14.14.3	0-4°C, immobilized enzyme, 0.1 mM dithiothreitol, 20% loss of activity in 3 days	Vibrio harveyi

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.14.14.3	decanal + FMNH + O2	-	Vibrio harveyi	decanoic acid + FMN + H2O + light	-	ir
1.14.14.3	dodecanal + FMNH + O2	-	Vibrio harveyi	dodecanoic acid + FMN + H2O + light	-	ir
1.14.14.3	octanal + FMNH + O2	-	Vibrio harveyi	octanoic acid + FMN + H2O + light	-	ir

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)