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Literature summary extracted from

  • Matsushita, K.; Ohno, Y.; Shinagawa, E.; Adachi, O.; Ameyama, M.
    Gluconsäure bildende Enzyme bei Aspergillus niger (1980), Agric. Biol. Chem., 44, 1505-1512.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.5.9 p-benzoquinone competitive inhibition of the activity with 2,6-dichlorophenolindophenol or coenzyme Q1, non-competitive inhibition of reaction with phenazine methosulfate Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.9 0.06
-
 coenzyme Q1
-
 Pseudomonas sp.
1.1.5.9 0.13
-
 phenazine methosulfate
-
 Pseudomonas sp.
1.1.5.9 0.47
-
 D-glucose reaction with 2,6-dichlorophenolindophenol at pH 6.0 Pseudomonas sp.
1.1.5.9 0.56
-
 tetramethyl-p-phenylenediamine
-
 Pseudomonas sp.
1.1.5.9 0.69
-
 ferricyanide
-
 Pseudomonas sp.
1.1.5.9 1.6
-
 2,6-dichlorophenolindophenol
-
 Pseudomonas sp.
1.1.5.9 6.3
-
 D-glucose reaction with phenazine methosulfate/2,6-dichlorophenolindophenol at pH 8.75 Pseudomonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.9 cytoplasmic membrane bound to Pseudomonas sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.9 87000
-
 1 * 87000, monomeric in presence of 1% Triton X-100, aggregation after removing the detergent, urea-SDS-PAGE Pseudomonas sp.
1.1.5.9 93000
-
 sucrose density gradient centrifugation, in presence of Triton X-100 Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.9 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.5.9
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.5.9 additional information
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.9 D-galactose + acceptor 6.5% of the activity with D-glucose Pseudomonas sp. D-galactono-1,5-lactone + reduced acceptor
-
 ?
1.1.5.9 D-glucose + 2,6-dichlorophenolindophenol
-
 Pseudomonas sp. D-gluconic acid + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.5.9 D-glucose + coenzyme Q1
-
 Pseudomonas sp. D-glucono-1,5-lactone + reduced coenzyme Q1
-
 ?
1.1.5.9 D-glucose + ferricyanide
-
 Pseudomonas sp. D-glucono-1,5-lactone + ferrocyanide
-
 ?
1.1.5.9 D-glucose + phenazine methosulfate
-
 Pseudomonas sp. D-glucono-1,5-lactone + reduced phenazine methosulfate
-
 ?
1.1.5.9 D-glucose + tetramethyl-p-phenylenediamine
-
 Pseudomonas sp. D-glucono-1,5-lactone + reduced tetramethyl-p-phenylenediamine
-
 ?
1.1.5.9 D-mannose + acceptor 8.6% of the activity with D-glucose Pseudomonas sp. D-manno-1,5-lactone + reduced acceptor
-
 ?
1.1.5.9 D-xylose + acceptor 13% of the activity with D-glucose Pseudomonas sp. D-xylono-1,5-lactone + reduced acceptor
-
 ?
1.1.5.9 L-arabinose + acceptor 2.8% of the activity with D-glucose Pseudomonas sp. L-arabinono-1,5-lactone + reduced acceptor
-
 ?
1.1.5.9 L-rhamnose + acceptor 7.5% of the activity with D-glucose Pseudomonas sp. L-rhamnone-1,5-lactone + reduced acceptor
-
 ?
1.1.5.9 maltose + acceptor 3.2% of the activity with D-glucose Pseudomonas sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.5.9 monomer 1 * 87000, monomeric in presence of 1% Triton X-100, aggregation after removing the detergent, urea-SDS-PAGE Pseudomonas sp.
1.1.5.9 More aggregation in medium containing no Triton X-100 to dimers, trimers and tetramers Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.9 4.5
-
 reaction with ferricyanide Pseudomonas sp.
1.1.5.9 6
-
 reaction with 2,6-dichlorophenolindophenol Pseudomonas sp.
1.1.5.9 6.5
-
 reaction with coenzyme Q1 Pseudomonas sp.
1.1.5.9 8.8
-
 reaction with phenazine methosulfate or tetramethyl-p-phenylenediamine Pseudomonas sp.