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Literature summary extracted from

  • Bruce, N.C.; Cain, R.B.; Pieper, D.H.; Engesser, K.H.
    Purification and characterization of 4-methylmuconolactone methyl-isomerase, a novel enzyme of the modified 3-oxoadipate pathway in nocardioform actinomycetes (1989), Biochem. J., 262, 303-312.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.99.14 (+)-muconolactone
-
 Rhodococcus rhodochrous
5.4.99.14 (-)1-Methyl-8-bromobislactone weak Rhodococcus rhodochrous
5.4.99.14 2-Bromo-4-methylbutan-1,4-olide weak Rhodococcus rhodochrous
5.4.99.14 2-Bromobutan-1,4-olide
-
 Rhodococcus rhodochrous
5.4.99.14 2-Methylbutan-1,4-olide
-
 Rhodococcus rhodochrous
5.4.99.14 4-Methylbut-3-en-1,4-olide angelicalactone Rhodococcus rhodochrous
5.4.99.14 4-methylbutan-1,4-olide
-
 Rhodococcus rhodochrous
5.4.99.14 AgNO3
-
 Rhodococcus rhodochrous
5.4.99.14 CuSO4
-
 Rhodococcus rhodochrous
5.4.99.14 HgCl2
-
 Rhodococcus rhodochrous
5.4.99.14 additional information EDTA Rhodococcus rhodochrous
5.4.99.14 PCMB
-
 Rhodococcus rhodochrous

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.14 0.17
-
 1-methyl-bislactone
-
 Rhodococcus rhodochrous
5.4.99.14 0.23
-
 4-carboxymethyl-4-methylbut-2-en-1,4-olide
-
 Rhodococcus rhodochrous

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.4.99.14 soluble
-
 Rhodococcus rhodochrous
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.99.14 17000
-
 4 * 17000, Rhodococcus rhodochrous, SDS-PAGE Rhodococcus rhodochrous
5.4.99.14 75500
-
 Rhodococcus rhodochrous, gel filtration Rhodococcus rhodochrous

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.14 Rhodococcus rhodochrous
-
 N75
-
5.4.99.14 Rhodococcus rhodochrous N75
-
 N75
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.14 N75 Rhodococcus rhodochrous

Reaction

EC Number Reaction Comment Organism Reaction ID
5.4.99.14 4-Carboxymethyl-4-methylbut-2-en-1,4-olide = 4-carboxymethyl-3-methylbut-2-en-1,4-olide isomerization does not involve actual methyl migration, but proceeds via the 1-methylbislactone Rhodococcus rhodochrous
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.4.99.14 culture condition:p-toluate-grown cell
-
 Rhodococcus rhodochrous
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.99.14 785
-
-
 Rhodococcus rhodochrous

Storage Stability

EC Number Storage Stability Organism
5.4.99.14 -30°C, slow loss of activity on prolonged storage Rhodococcus rhodochrous

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.14 1-Methylbislactone
-
 Rhodococcus rhodochrous 4-Carboxymethyl-3-methylbut-2-en-1,4-olide
-
 ?
5.4.99.14 1-Methylbislactone
-
 Rhodococcus rhodochrous N75 4-Carboxymethyl-3-methylbut-2-en-1,4-olide
-
 ?
5.4.99.14 4-Carboxymethyl-4-methylbut-2-en-1,4-olide highly specific, accepts only (+)-(4S)-4-methylmuconolactone, i.e. 4-carboxymethyl-4-methylbut-2-en-1,4-olide and the putative reaction intermediate 1-methylbislactone Rhodococcus rhodochrous 4-Carboxymethyl-3-methylbut-2-en-1,4-olide i.e. (-)-(4S)-3-methylmuconolactone ?
5.4.99.14 4-Carboxymethyl-4-methylbut-2-en-1,4-olide highly specific, accepts only (+)-(4S)-4-methylmuconolactone, i.e. 4-carboxymethyl-4-methylbut-2-en-1,4-olide and the putative reaction intermediate 1-methylbislactone Rhodococcus rhodochrous N75 4-Carboxymethyl-3-methylbut-2-en-1,4-olide i.e. (-)-(4S)-3-methylmuconolactone ?

Subunits

EC Number Subunits Comment Organism
5.4.99.14 tetramer 4 * 17000, Rhodococcus rhodochrous, SDS-PAGE Rhodococcus rhodochrous

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.99.14 30
-
 assay at Rhodococcus rhodochrous

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.99.14 7
-
-
 Rhodococcus rhodochrous

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.4.99.14 6 8.5 active in this range, rapid decrease of activity above pH 8.5 Rhodococcus rhodochrous