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Literature summary extracted from

  • Cunane, L.M.; Barton, J.D.; Chen, Z.W.; Welsh, F.E.; Chapman, S.K.; Reid, G.A.; Mathews, F.S.
    Crystallographic study of the recombinant flavin-binding domain of baker's yeast flavocytochrome b(2): comparison with the intact wild-type enzyme (2002), Biochemistry, 41, 4264-4272.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.2.3 in Escherichia coli TG1 Saccharomyces cerevisiae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.2.3 crystallization of flavin binding domain and intact enzyme, hanging drop vapor diffusion method Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
1.1.2.3 K201K mutant Saccharomyces cerevisiae
1.1.2.3 L199E mutant Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.2.3 Fe2+ bound to enzyme Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.2.3 57500
-
 4 * 57500, SDS-PAGE Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.2.3 (S)-lactate + 2 ferricytochrome c Saccharomyces cerevisiae can feed electrons to respiratory chain at the level of cytochrome c pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.3 Saccharomyces cerevisiae P00175
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.2.3
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.3 (S)-lactate + 2 ferricytochrome c
-
 Saccharomyces cerevisiae pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?
1.1.2.3 (S)-lactate + 2 ferricytochrome c can feed electrons to respiratory chain at the level of cytochrome c Saccharomyces cerevisiae pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?
1.1.2.3 additional information ferricyanide used as electron acceptor Saccharomyces cerevisiae ?
-
 ?
1.1.2.3 additional information electron acceptors other than ferricytochrome c used Saccharomyces cerevisiae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.2.3 tetramer 4 * 57500, SDS-PAGE Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.3 FMN one FMN per subunit Saccharomyces cerevisiae
1.1.2.3 heme one heme per subunit Saccharomyces cerevisiae
1.1.2.3 additional information ferricyanide used as electron acceptor Saccharomyces cerevisiae
1.1.2.3 additional information electron acceptors other than ferricytochrome c used Saccharomyces cerevisiae