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Literature summary extracted from

  • Gondry, M.; Duboist, J.; Terrier, M.; Lederer, F.
    The catalytic role of tyrosine 254 in flavocytochrome b2 (L-lactate dehydrogenase from baker's yeast). Comparison between the Y254F and Y254L mutant proteins (2001), Eur. J. Biochem., 268, 4918-4927.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.2.3 in Escherichia coli MM294 Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
1.1.2.3 Y254F only traces of activity Saccharomyces cerevisiae
1.1.2.3 Y254L turnover reduced to less than 10% Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.2.3 D-lactate
-
 Saccharomyces cerevisiae
1.1.2.3 L-Mandelate competitive Saccharomyces cerevisiae
1.1.2.3 oxalate
-
 Saccharomyces cerevisiae
1.1.2.3 pyruvate competitive at low concentrations, non-competitve at high concentrations Saccharomyces cerevisiae
1.1.2.3 sulfite competitive Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.2.3 mitochondrial intermembrane space
-
 Saccharomyces cerevisiae 5758
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.2.3 Fe2+ bound to enzyme Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.2.3 (S)-lactate + 2 ferricytochrome c Saccharomyces cerevisiae can feed electrons to respiratory chain at the level of cytochrome c pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.3 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.2.3
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.3 (S)-lactate + 2 ferricytochrome c
-
 Saccharomyces cerevisiae pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?
1.1.2.3 (S)-lactate + 2 ferricytochrome c can feed electrons to respiratory chain at the level of cytochrome c Saccharomyces cerevisiae pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?
1.1.2.3 additional information ferricyanide used as electron acceptor Saccharomyces cerevisiae ?
-
 ?
1.1.2.3 additional information electron acceptors other than ferricytochrome c used Saccharomyces cerevisiae ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.3 additional information ferricyanide used as electron acceptor Saccharomyces cerevisiae
1.1.2.3 additional information electron acceptors other than ferricytochrome c used Saccharomyces cerevisiae