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Literature summary extracted from

  • Tegoni, M.; Begotti, S.; Cambillau, C.
    X-ray structure of two complexes of the Y143F flavocytochrome b2 mutant crystallized in the presence of lactate or phenyl lactate (1995), Biochemistry, 34, 9840-9850.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.2.3 in Escherichia coli Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
1.1.2.3 Y143F
-
 Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.2.3 0.23
-
 L-lactate Y143F mutant with cytochrome c as electron acceptor Saccharomyces cerevisiae
1.1.2.3 0.24
-
 L-lactate native enzyme with cytochrome c as electron acceptor Saccharomyces cerevisiae
1.1.2.3 0.49
-
 L-lactate native enzyme mutant with ferricyanide as electron acceptor Saccharomyces cerevisiae
1.1.2.3 2.9
-
 L-lactate Y143F mutant with ferricyanide as electron acceptor Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.2.3 mitochondrial intermembrane space soluble Saccharomyces cerevisiae 5758
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.2.3 Fe2+ bound to enzyme Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.2.3 (S)-lactate + 2 ferricytochrome c Saccharomyces cerevisiae can feed electrons to respiratory chain at the level of cytochrome c pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.3 Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.2.3
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
1.1.2.3 4°C, precipitate from 70% saturated ammonium sulfate, under nitrogen, stable for several weeks Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.3 (S)-lactate + 2 ferricytochrome c
-
 Saccharomyces cerevisiae pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?
1.1.2.3 (S)-lactate + 2 ferricytochrome c can feed electrons to respiratory chain at the level of cytochrome c Saccharomyces cerevisiae pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?
1.1.2.3 additional information ferricyanide used as electron acceptor Saccharomyces cerevisiae ?
-
 ?
1.1.2.3 additional information electron acceptors other than ferricytochrome c used Saccharomyces cerevisiae ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.2.3 11
-
 L-lactate Y143F mutant with cytochrome c as electron acceptor Saccharomyces cerevisiae
1.1.2.3 103
-
 L-lactate native enzyme with cytochrome c as electron acceptor Saccharomyces cerevisiae
1.1.2.3 400
-
 L-lactate native enzyme and Y143F mutant with ferricyanide as electron acceptor Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.3 FMN
-
 Saccharomyces cerevisiae
1.1.2.3 heme
-
 Saccharomyces cerevisiae
1.1.2.3 additional information ferricyanide used as electron acceptor Saccharomyces cerevisiae
1.1.2.3 additional information electron acceptors other than ferricytochrome c used Saccharomyces cerevisiae