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Literature summary extracted from

  • Silvestrini, M.C.; Tegoni, M.; Celerier, J.; Desbois, A.; Gervais, M.
    Expression in Escherichia coli of the flavin and the haem domains of Hansenula anomala flavocytochrome b2 (flavodehydrogenase and b2 core) and characterization of the recombinant proteins (1993), Biochem. J., 295, 501-508.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.2.3 flavin and heme domain expressed in Escherichia coli Wickerhamomyces anomalus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.2.3 L-lactate 40% inhibition at 60 mM Wickerhamomyces anomalus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.2.3 3
-
 L-lactate with ferricyanide as electron acceptor Wickerhamomyces anomalus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.2.3 Fe2+ bound to enzyme Wickerhamomyces anomalus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.2.3 (S)-lactate + 2 ferricytochrome c Wickerhamomyces anomalus
-
 pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.3 Wickerhamomyces anomalus
-
 flavin and heme domain expressed in Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.2.3 flavin and heme domain from cloned Escherichia coli Wickerhamomyces anomalus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.3 (S)-lactate + 2 ferricytochrome c
-
 Wickerhamomyces anomalus pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?
1.1.2.3 additional information ferricyanide used as electron acceptor Wickerhamomyces anomalus ?
-
 ?
1.1.2.3 additional information electron acceptors other than ferricytochrome c used Wickerhamomyces anomalus ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.3 additional information ferricyanide used as electron acceptor Wickerhamomyces anomalus
1.1.2.3 additional information electron acceptors other than ferricytochrome c used Wickerhamomyces anomalus