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Literature summary extracted from

  • Pritchard, G.G.
    An NAD+-independent L-lactate dehydrogenase from Rhizopus oryzae (1971), Biochim. Biophys. Acta, 250, 25-34.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.2.3 oxalate complete inhibition, competitive Rhizopus arrhizus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.2.3 3.85
-
 L-lactate 2,6-dichlorophenolindopenol as electron acceptor Rhizopus arrhizus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.2.3 Fe2+ bound to enzyme Rhizopus arrhizus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.2.3 (S)-lactate + 2 ferricytochrome c Rhizopus arrhizus replaces activity of EC 1.1.1.27 in limited substrate conditions pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.3 Rhizopus arrhizus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.2.3 partial Rhizopus arrhizus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.2.3 0.055
-
 (S)-lactate with cytochrome c as electron acceptor Rhizopus arrhizus
1.1.2.3 0.415
-
 partially purified enzyme with 2,6-dichloroindophenol as electron acceptor Rhizopus arrhizus
1.1.2.3 0.72
-
 6-dichloroindophenol as electron acceptor Rhizopus arrhizus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.3 (S)-lactate + 2 ferricytochrome c D-isomer not oxidized Rhizopus arrhizus pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?
1.1.2.3 (S)-lactate + 2 ferricytochrome c replaces activity of EC 1.1.1.27 in limited substrate conditions Rhizopus arrhizus pyruvate + 2 ferrocytochrome c + 2 H+
-
 ?
1.1.2.3 additional information 2,6-dichlorophenolindophenol, ferricyanide and cytochrome c but not oxygen can serve as electron acceptor, rate of reduction with cytochrome c much slower than with the other possible acceptors, glycolate and L-malate do not serve as substrates Rhizopus arrhizus ?
-
 ?
1.1.2.3 additional information electron acceptors other than ferricytochrome c used Rhizopus arrhizus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.2.3 7.2 8.4
-
 Rhizopus arrhizus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.2.3 5.2
-
 almost no activity Rhizopus arrhizus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.3 cytochrome c
-
 Rhizopus arrhizus
1.1.2.3 additional information electron acceptors other than ferricytochrome c used Rhizopus arrhizus
1.1.2.3 additional information NAD+ and NADP+ cannot substitute, 2,6-dichlorophenolindophenol, ferricyanide and cytochrome c but not oxygen can serve as electron acceptor, rate of reduction with cytochrome c much slower than with the other possible acceptors Rhizopus arrhizus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.2.3 0.4
-
 oxalate 10 mM Rhizopus arrhizus
1.1.2.3 0.445
-
 oxalate 1 mM Rhizopus arrhizus