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Literature summary extracted from

  • Wyrambik, D.; Grisebach, H.
    Purification and properties of isoenzymes of cinnamyl-alcohol dehydrogenase from soybean-cell-suspension cultures (1975), Eur. J. Biochem., 59, 9-15.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.194 0.0074
-
 NADP+
-
 Glycine max
1.1.1.194 0.83
-
 coniferyl alcohol
-
 Glycine max
1.1.1.195 0.0017
-
 coniferaldehyde
-
 Glycine max
1.1.1.195 0.0025
-
 cinnamaldehyde
-
 Glycine max
1.1.1.195 0.0038
-
 NADP+
-
 Glycine max
1.1.1.195 0.0043
-
 sinapaldehyde
-
 Glycine max
1.1.1.195 0.0069
-
 3,4-dimethoxycinnamaldehyde
-
 Glycine max
1.1.1.195 0.0072
-
 NADPH
-
 Glycine max
1.1.1.195 0.0091
-
 p-coumaraldehyde
-
 Glycine max
1.1.1.195 0.011
-
 coniferyl alcohol
-
 Glycine max
1.1.1.195 0.022
-
 3,4-dimethoxycinnamyl alcohol
-
 Glycine max
1.1.1.195 0.026
-
 cinnamyl alcohol
-
 Glycine max
1.1.1.195 0.05
-
 sinapyl alcohol
-
 Glycine max
1.1.1.195 0.057
-
 p-coumaryl alcohol
-
 Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.194 43000
-
 gel filtration Glycine max
1.1.1.195 69000
-
 gel filtration Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.194 coniferyl alcohol + NADP+ Glycine max
-
 coniferyl aldehyde + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.194 Glycine max
-
 L. var. Mandarin
-
1.1.1.195 Glycine max
-
 var. Mandarin
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.194 var. Mandarin Glycine max
1.1.1.195 var. Mandarin Glycine max

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.194 cell suspension culture
-
 Glycine max
-
1.1.1.195 cell suspension culture
-
 Glycine max
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.194 0.36
-
-
 Glycine max
1.1.1.195 3.86
-
-
 Glycine max

Storage Stability

EC Number Storage Stability Organism
1.1.1.194 -20°C, 42 mM 2-mercaptoethanol, 10% ethyleneglycol, several weeks Glycine max
1.1.1.195 -20°C, 42 mM 2-mercaptoethanol, 10% ethylene glycol, 5 months Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.194 coniferyl alcohol + NADP+
-
 Glycine max coniferyl aldehyde + NADPH + H+
-
 ?
1.1.1.195 3,4-dimethoxycinnamyl alcohol + NADP+
-
 Glycine max 3,4-dimethoxycinnamaldehyde + NADPH
-
 r
1.1.1.195 cinnamyl alcohol + NADP+
-
 Glycine max cinnamaldehyde + NADPH
-
 r
1.1.1.195 coniferyl alcohol + NADP+
-
 Glycine max coniferyl aldehyde + NADPH + H+
-
 r
1.1.1.195 p-coumaryl alcohol + NADP+
-
 Glycine max p-coumaraldehyde + NADPH
-
 r
1.1.1.195 sinapyl alcohol + NADP+
-
 Glycine max sinapaldehyde + NADPH
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.194 9.2
-
 coniferyl alcohol oxidation Glycine max
1.1.1.195 6.5
-
 coniferyl aldehyde reduction Glycine max
1.1.1.195 8.8
-
 coniferyl alcohol oxidation Glycine max

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.195 6 8 coniferyl aldehyde reduction, pH 6.0: about 70% of activity maximum, pH 8.0: about 30% of activity maximum Glycine max
1.1.1.195 7.2 9.7 coniferyl alcohol oxidation, pH 7.2: about 40% of activity maximum, pH 9.7: about 35% of activity maximum Glycine max

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.194 additional information no reaction with NAD+ or NADH Glycine max
1.1.1.194 NADP+
-
 Glycine max
1.1.1.194 NADPH
-
 Glycine max
1.1.1.195 NADP+
-
 Glycine max
1.1.1.195 NADPH
-
 Glycine max