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Literature summary extracted from

  • Nagashima, H.; Inoue, J.; Sasaki, E.; Yamamoto, S.; Sasaki, Y.; Yamauchi-Inomata, Y.; Harayama, S.
    Long-chain n-alkanol dehydrogenase from Pseudomonas putida (1996), J. Ferment. Bioeng., 82, 328-333.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.192 0.044
-
 n-butanol
-
 Pseudomonas putida
1.1.1.192 0.053
-
 n-Propanol
-
 Pseudomonas putida
1.1.1.192 0.095
-
 n-decanal
-
 Pseudomonas putida
1.1.1.192 0.14
-
 n-Hexanol
-
 Pseudomonas putida
1.1.1.192 0.17
-
 NADH
-
 Pseudomonas putida
1.1.1.192 0.18
-
 n-Pentanol
-
 Pseudomonas putida
1.1.1.192 0.23
-
 n-decanol
-
 Pseudomonas putida
1.1.1.192 0.26
-
 n-Heptanol
-
 Pseudomonas putida
1.1.1.192 0.31
-
 NAD+
-
 Pseudomonas putida
1.1.1.192 0.34
-
 n-Octanol
-
 Pseudomonas putida
1.1.1.192 0.36
-
 n-nonanol
-
 Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.192 Zinc 1.1 mol per mol of subunit Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.192 40000
-
 4 * 40000, SDS-PAGE Pseudomonas putida
1.1.1.192 163000
-
 gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.192 Pseudomonas putida
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.192
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.192 long-chain alcohol + NAD+ + H2O long chain n-alcanols up to C12 Pseudomonas putida long-chain carboxylate + NADH
-
 r
1.1.1.192 n-butanol + NAD+ + H2O
-
 Pseudomonas putida n-butanal + NADH
-
 ?
1.1.1.192 n-decanol + NAD+ + H2O
-
 Pseudomonas putida n-decanal + NADH
-
 r
1.1.1.192 n-heptanol + NAD+ + H2O
-
 Pseudomonas putida n-heptanal + NADH
-
 ?
1.1.1.192 n-hexanol + NAD+ + H2O
-
 Pseudomonas putida n-hexanal + NADH
-
 ?
1.1.1.192 n-nonanol + NAD+ + H2O
-
 Pseudomonas putida n-nonanal + NADH
-
 ?
1.1.1.192 n-octanol + NAD+ + H2O
-
 Pseudomonas putida n-octanal + NADH
-
 ?
1.1.1.192 n-pentanol + NAD+ + H2O
-
 Pseudomonas putida n-pentanal + NADH
-
 ?
1.1.1.192 n-propanol + NAD+ + H2O
-
 Pseudomonas putida n-propanal + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.192 More N-terminal sequence Pseudomonas putida
1.1.1.192 tetramer 4 * 40000, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.192 50
-
-
 Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.192 50
-
 stable up to Pseudomonas putida
1.1.1.192 70
-
 10 min, all activity lost Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.192 7
-
 n-decanol, reduction of Pseudomonas putida
1.1.1.192 9.5
-
 n-decanol, oxidation of Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.192 NAD+ absolute requirement Pseudomonas putida