EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.14.15.1 | Pseudomonas putida PpG786, cytochrome m | Pseudomonas putida |
EC Number | General Stability | Organism |
---|---|---|
1.14.15.1 | 2-mercaptoethanol retards loss of FeS-chromophore from putidaredoxin during purification | Pseudomonas putida |
1.14.15.1 | glycerol, minimizes loss of FMN during purification of putidaredoxin reductase | Pseudomonas putida |
1.14.15.1 | multiple freezing and thawing, at -20°C, cytochrome m accumulates an equally active, heme-containing component of higher molecular weight, DTT reconverts it to native cytochrome m at 25°C | Pseudomonas putida |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.15.1 | cytoplasm | - |
Pseudomonas putida | 5737 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.15.1 | Fe2+ | requirement, enzyme complex with two FeS-protein components | Pseudomonas putida | |
1.14.15.1 | K+ | increase of activity, selective effector of enhanced substrate affinity to cytochrome m | Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.15.1 | additional information | - |
putidaredoxin reductase: MW 45000 Da, putidaredoxin: MW 11000 Da, cytochrome m: MW 44000 Da, Pseudomonas putida PpG786, gel filtration | Pseudomonas putida |
1.14.15.1 | additional information | - |
putidaredoxin reductase, MW 43500 Da, putidaredoxin, MW 11594 Da, cytochrome m, MW 45000 Da, Pseudomonas putida PpG786, analytical data | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.15.1 | (+)-camphor + putidaredoxin + O2 | Pseudomonas putida | - |
(R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | i.e. (+)-exo-5-hydroxycamphor | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.15.1 | Pseudomonas putida | - |
PpG786, mutant derived from PpG1 | - |
1.14.15.1 | Pseudomonas putida | - |
Accession Number PC00183 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.15.1 | PpG786 | Pseudomonas putida |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.15.1 | 30.26 | - |
putidaredoxin reductase | Pseudomonas putida |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.15.1 | -196°C, no loss of activity of putidaredoxin reductase after repeated freeze-thaw cycles | Pseudomonas putida |
1.14.15.1 | 0°C, putidaredoxin slowly loses its prosthetic group, 2-mercaptoethanol retards apoprotein formation | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.15.1 | (+)-camphor + putidaredoxin + O2 | - |
Pseudomonas putida | (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | i.e. (+)-exo-5-hydroxycamphor | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.15.1 | More | multi-component mixed function monooxygenase consisting of putidaredoxin reductase, MW 47000, putidaredoxin, MW 11700, cytochrome m, MW 50500, Pseudomonas putida PpG786, SDS-PAGE | Pseudomonas putida |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.15.1 | 25 | - |
assay at | Pseudomonas putida |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.15.1 | 2 | - |
putidaredoxin | constituent protein of the multi-component oxygenase | Pseudomonas putida | |
1.14.15.1 | 34 | - |
cytochrome m | P-450cam, constituent part of the multi-component enzyme | Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.15.1 | 7 | - |
assay at | Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.15.1 | cytochrome m | cytochrome P-450cam, essential, b-type heme-thiolate protein of P-450-class | Pseudomonas putida | |
1.14.15.1 | FMN | - |
Pseudomonas putida | |
1.14.15.1 | NADH | - |
Pseudomonas putida | |
1.14.15.1 | putidaredoxin | essential, iron-sulfur redox protein, Fe2S2*Cys4-class, e-transfer agent to and effector of cytochrome P450cam | Pseudomonas putida |