EC Number | General Stability | Organism |
---|---|---|
1.14.15.1 | freeze-thawing, less than 5% loss of activity of cytochrome P450cam in the presence of camphor | Pseudomonas putida |
1.14.15.1 | putidaredoxin suffers degradation by repeated cycles of freezing and thawing | Pseudomonas putida |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.15.1 | cytoplasm | - |
Pseudomonas putida | 5737 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.15.1 | Fe2+ | requirement, enzyme complex with two FeS-protein components | Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.15.1 | additional information | - |
putidaredoxin reductase: MW 43000 Da, putidaredoxin: MW 12500 Da, cytochrome P450cam: MW 45000 Da, Pseudomonas putida PpG786, ultracentrifugal, diffusion and amino acid analysis | Pseudomonas putida |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.15.1 | (+)-camphor + putidaredoxin + O2 | Pseudomonas putida | - |
(R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | i.e. (+)-exo-5-hydroxycamphor | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.15.1 | Pseudomonas putida | - |
PpG786, mutant derived from PpG1 | - |
1.14.15.1 | Pseudomonas putida | - |
Accession Number PC00183 | - |
EC Number | Oxidation Stability | Organism |
---|---|---|
1.14.15.1 | under aerobic conditions, cytochrome P450cam decays at 25°C with t1/2 of 180 min to cytochrome P420, not rapidly in the presence of camphor | Pseudomonas putida |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.15.1 | PpG786 | Pseudomonas putida |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.14.15.1 | (+)-camphor + reduced putidaredoxin + O2 = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | multi-component mixed function oxidase, consisting of putidaredoxin reductase, putidaredoxin and cytochrome P450cam, heme-thiolate protein | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.15.1 | (+)-camphor + putidaredoxin + O2 | - |
Pseudomonas putida | (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | i.e. (+)-exo-5-hydroxycamphor | ? | |
1.14.15.1 | (+)-camphor + putidaredoxin + O2 | the (+)- and (-)-enantiomers serve as substrates | Pseudomonas putida | (R)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O | i.e. (+)-exo-5-hydroxycamphor | ? | |
1.14.15.1 | 1,2-campholide + putidaredoxin + O2 | - |
Pseudomonas putida | 5-exo-hydroxy-1,2-campholide + oxidized putidaredoxin + H2O | - |
? | |
1.14.15.1 | additional information | - |
Pseudomonas putida | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.15.1 | 25 | - |
assay at | Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.15.1 | 7.4 | - |
assay at | Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.15.1 | cytochrome m | - |
Pseudomonas putida | |
1.14.15.1 | FMN | - |
Pseudomonas putida | |
1.14.15.1 | NADH | requirement, reduces reductase flavoprotein and putidaredoxin, but not P450cam, in the absence of camphor | Pseudomonas putida | |
1.14.15.1 | putidaredoxin | cannot be replaced by other FeS-proteins or the phospholipid of the hepatic microsomal P450 system | Pseudomonas putida |