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Literature summary extracted from

  • Gentry-Weeks, C.R.; Keith, J.M.; Thompson, J.
    Toxicity of Bordetella avium beta-cystathionase toward MC3T3-E1 osteogenic cells (1993), J. Biol. Chem., 268, 7298-7314.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.13 expression in Escherichia coli Bordetella avium

General Stability

EC Number General Stability Organism
4.4.1.13 no decrease in activity after repeated freezing, -20°C, and thawing Bordetella avium

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.13 DTT 10 mM, 19% inhibition Bordetella avium
4.4.1.13 hydroxylamine 1 mM, 92% inhibition Bordetella avium
4.4.1.13 iodoacetamide 10 mM, 61% inhibition Bordetella avium
4.4.1.13 iodoacetate 10 mM, 25% inhibition Bordetella avium
4.4.1.13 KCN 1 mM, 84% inhibition Bordetella avium
4.4.1.13 L-canaline 1 mM, 67% inhibition, 10 mM complete inhibition Bordetella avium
4.4.1.13 NEM 1 mM, 94% inhibition Bordetella avium
4.4.1.13 p-hydroxymercuribenzoate 1 mM, 92% inhibition Bordetella avium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.13 0.033
-
 L-djenkolate
-
 Bordetella avium
4.4.1.13 0.07
-
 L-cystathionine
-
 Bordetella avium
4.4.1.13 0.104
-
 Lanthionine
-
 Bordetella avium
4.4.1.13 0.47
-
 S-(2-aminoethyl)-L-Cys
-
 Bordetella avium
4.4.1.13 0.67
-
 S-methyl-L-Cys
-
 Bordetella avium
4.4.1.13 5.3
-
 D-Cys
-
 Bordetella avium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.13 41000
-
 2 * 41000, SDS-PAGE Bordetella avium
4.4.1.13 42606
-
 2 * 42606, calculation from nucleotide sequence Bordetella avium
4.4.1.13 80000
-
 gel filtration Bordetella avium

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.13 Bordetella avium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.13
-
 Bordetella avium

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Storage Stability

EC Number Storage Stability Organism
4.4.1.13 -20°C, stable for 6 months Bordetella avium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.13 D-Cys + H2O
-
 Bordetella avium ?
-
 ?
4.4.1.13 L-cystathionine + H2O
-
 Bordetella avium L-homocysteine + NH3 + pyruvate
-
 ?
4.4.1.13 L-cystine + H2O
-
 Bordetella avium L-thiocysteine + pyruvate + NH3
-
 ?
4.4.1.13 L-djenkolate + H2O
-
 Bordetella avium pyruvate
-
 ?
4.4.1.13 lanthionine + H2O
-
 Bordetella avium L-cysteine + NH3 + pyruvate
-
 ?
4.4.1.13 additional information osteotoxin is lethal for MC3T3-E1 osteogenic cells, fetal bovine trabecular cells, UMR106-01(BSP) rat osteosarcoma cells and embryonic bovine tracheal cells. The cytotoxicity can be attributed to: 1. beta-cystathionase-catalyzed cleavage of L-cystine in the medium and formation of reactive sulfane-containing derivatives, 2. Transfer of sulfane sulfur to metabolically sensitive or structurally important proteins in the osteogenic cells Bordetella avium ?
-
 ?
4.4.1.13 S-(2-aminoethyl)-L-Cys + H2O
-
 Bordetella avium ?
-
 ?
4.4.1.13 S-methyl-L-Cys + H2O
-
 Bordetella avium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.13 dimer 2 * 41000, SDS-PAGE Bordetella avium
4.4.1.13 dimer 2 * 42606, calculation from nucleotide sequence Bordetella avium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.4.1.13 20
-
 room temperature, t1/2: 24 min Bordetella avium
4.4.1.13 25 40 stable, rapid decline in activity above 40°C Bordetella avium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.13 8 9
-
 Bordetella avium

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.13 pyridoxal 5'-phosphate each subunit of the dimer contains one molecule of pyridoxal 5'-phosphate Bordetella avium