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Literature summary extracted from

  • Ravanel, S.; Job, D.; Douce, R.
    Purification and properties of cystathionine beta-lyase from Arabidopsis thaliana overexpressed in Escherichia coli (1996), Biochem. J., 320, 383-392.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.13 overexpression in Escherichia coli Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.13 aminoethoxyvinylglycine irreversible Arabidopsis thaliana
4.4.1.13 iodoacetamide
-
 Arabidopsis thaliana
4.4.1.13 L-alpha-(2-aminoethoxyvinyl)glycine
-
 Arabidopsis thaliana
4.4.1.13 NEM
-
 Arabidopsis thaliana
4.4.1.13 rhizobitoxine
-
 Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.13 46000
-
 4 * 46000, SDS-PAGE Arabidopsis thaliana
4.4.1.13 170000
-
 gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.13 cystathionine + H2O Arabidopsis thaliana enzyme is involved in methionine biosynthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.13 Arabidopsis thaliana
-
 var. columbia
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.13
-
 Arabidopsis thaliana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.13 24.5
-
-
 Arabidopsis thaliana

Storage Stability

EC Number Storage Stability Organism
4.4.1.13 -80°C, stable for several months Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.13 cystathionine + H2O enzyme is involved in methionine biosynthesis Arabidopsis thaliana ?
-
 ?
4.4.1.13 L-cystathionine + H2O
-
 Arabidopsis thaliana L-homocysteine + NH3 + pyruvate
-
 ?
4.4.1.13 L-djenkolate + H2O
-
 Arabidopsis thaliana pyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.13 tetramer 4 * 46000, SDS-PAGE Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.4.1.13 60
-
-
 Arabidopsis thaliana

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.4.1.13 60 90 60°C: optimum, 90°C: 17% of maximal activity Arabidopsis thaliana

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.4.1.13 60
-
 15 min, 90% loss of activity Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.13 8.5
-
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.13 pyridoxal 5'-phosphate each subunit of the tetramer is associated with one molecule of pyridoxal 5'-phosphate. Schiff-base formation between the cofactor and the holoenzyme occurs at Lys278 Arabidopsis thaliana