Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Droux, M.; Ravanel, S.; Douce, R.
    Methionine biosynthesis in higher plants. II. Purification and characterization of cystathionine beta-lyase from spinach chloroplasts (1995), Arch. Biochem. Biophys., 316, 585-595.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.13 DL-propargylglycine no inhibition Spinacia oleracea
4.4.1.13 L-alpha-(2-aminoethoxyvinyl)glycine
-
 Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.13 0.15
-
 L-cystathionine chloroplastic isoenzyme Spinacia oleracea
4.4.1.13 0.34
-
 L-djenkolate chloroplastic isoenzyme Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.4.1.13 chloroplast
-
 Spinacia oleracea 9507
-
4.4.1.13 cytosol
-
 Spinacia oleracea 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.13 44000
-
 4 * 44000, chloroplastic isoenzyme, SDS-PAGE Spinacia oleracea
4.4.1.13 170000
-
 chloroplastic isoenzyme, gel filtration Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.13 cystathionine + H2O Spinacia oleracea
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.13 Spinacia oleracea
-
 a cytosolic and a chloroplastic isoenzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.13 chloroplastic isoenzyme Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.4.1.13 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.13 additional information
-
-
 Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.13 cystathionine + H2O
-
 Spinacia oleracea ?
-
 ?
4.4.1.13 L-cystathionine + H2O
-
 Spinacia oleracea L-homocysteine + NH3 + pyruvate
-
 ?
4.4.1.13 L-djenkolate + H2O
-
 Spinacia oleracea pyruvate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.13 tetramer 4 * 44000, chloroplastic isoenzyme, SDS-PAGE Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.13 8.3 9 with L-cystathionine or L-djenkolate as substrate Spinacia oleracea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.4.1.13 7.5 9 pH 7.5: about 20% of maximal activity, pH 7.5-9.0: optimum Spinacia oleracea