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Literature summary extracted from

  • Giovanelli, J.
    Cystathionine beta-lyase from spinach (1987), Methods Enzymol., 143, 443-449.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.13 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Spinacia oleracea
4.4.1.13 Beta-cyanoalanine competitive; strong Spinacia oleracea
4.4.1.13 NEM
-
 Spinacia oleracea
4.4.1.13 rhizobitoxine active-site directed, irreversible Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.13 0.13
-
 L-cystathionine
-
 Spinacia oleracea
4.4.1.13 0.25
-
 L-djenkolate
-
 Spinacia oleracea

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.13 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.13
-
 Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.4.1.13 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.13 0.111
-
-
 Spinacia oleracea

Storage Stability

EC Number Storage Stability Organism
4.4.1.13 -80°C or at 4°C, 5 days, 70% loss of activity, partially purified enzyme Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.13 L-cystathionine + H2O no gamma-cleavage Spinacia oleracea L-homocysteine + NH3 + pyruvate
-
 ?
4.4.1.13 L-djenkolate + H2O
-
 Spinacia oleracea pyruvate
-
 ?
4.4.1.13 mixed disulfide of L-cysteine and L-homocysteine + H2O
-
 Spinacia oleracea ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.13 8.8
-
-
 Spinacia oleracea