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Literature summary extracted from

  • Kamitani, H.; Esaki, N.; Tanaka, H.; Soda, K.
    Thermostable S-alkylcysteine alpha, beta-lyase from a thermophile: purification and properties (1990), Agric. Biol. Chem., 54, 2069-2076.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.13 HgCl2 57% inhibition at 1 mM Bacillus sp. (in: Bacteria)
4.4.1.13 hydoxylamine complete inhibition at 1 mM Bacillus sp. (in: Bacteria)
4.4.1.13 p-chloromercuribenzoate 82% inhibition at 1 mM Bacillus sp. (in: Bacteria)
4.4.1.13 phenylhydrazine complete inhibition at 1 mM Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.13 0.73
-
 S-Benzyl-L-cysteine
-
 Bacillus sp. (in: Bacteria)
4.4.1.13 2
-
 L-selenodjenkolate
-
 Bacillus sp. (in: Bacteria)
4.4.1.13 3.6
-
 L-djenkolate
-
 Bacillus sp. (in: Bacteria)
4.4.1.13 4.6
-
 Se-(methyl)-L-selenocysteine
-
 Bacillus sp. (in: Bacteria)
4.4.1.13 4.8
-
 L-cysteine
-
 Bacillus sp. (in: Bacteria)
4.4.1.13 5.1
-
 S-methyl-L-cysteine
-
 Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.13 39000
-
 2 * 39000, SDS-PAGE Bacillus sp. (in: Bacteria)
4.4.1.13 76000
-
 gel filtration Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.13 Bacillus sp. (in: Bacteria)
-
 41A
-
4.4.1.13 Bacillus sp. (in: Bacteria) 41A
-
 41A
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.13
-
 Bacillus sp. (in: Bacteria)

Storage Stability

EC Number Storage Stability Organism
4.4.1.13 -20°C, 10 mM potassium phospate buffer, pH 7.2, with 50% glycerol, 0.01 mM pyridoxal 5'-phosphate and 0.01% 2-mercaptoethanol, 1 month without loss of activity Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.13 L-cysteine + H2O
-
 Bacillus sp. (in: Bacteria) pyruvate + NH3 + hydrogen sulfide
-
 ?
4.4.1.13 L-cysteine + H2O
-
 Bacillus sp. (in: Bacteria) 41A pyruvate + NH3 + hydrogen sulfide
-
 ?
4.4.1.13 L-djenkolate
-
 Bacillus sp. (in: Bacteria) pyruvate + NH3 + S-(mercaptomethyl)cysteine
-
 ?
4.4.1.13 L-djenkolate
-
 Bacillus sp. (in: Bacteria) 41A pyruvate + NH3 + S-(mercaptomethyl)cysteine
-
 ?
4.4.1.13 L-Selenodjenkolate
-
 Bacillus sp. (in: Bacteria) Se-(hydroselenomethyl)selenocysteine + pyruvate + NH3
-
 ?
4.4.1.13 L-Selenodjenkolate
-
 Bacillus sp. (in: Bacteria) 41A Se-(hydroselenomethyl)selenocysteine + pyruvate + NH3
-
 ?
4.4.1.13 S-benzyl-L-cysteine + H2O
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
4.4.1.13 S-benzyl-L-cysteine + H2O
-
 Bacillus sp. (in: Bacteria) 41A ?
-
 ?
4.4.1.13 S-methyl-L-cysteine
-
 Bacillus sp. (in: Bacteria) methyl mercaptane + pyruvate + NH3
-
 ?
4.4.1.13 S-methyl-L-cysteine
-
 Bacillus sp. (in: Bacteria) 41A methyl mercaptane + pyruvate + NH3
-
 ?
4.4.1.13 Se-(methyl)-L-selenocysteine
-
 Bacillus sp. (in: Bacteria) HSe(CH3) + pyruvate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.13 dimer 2 * 39000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.4.1.13 70
-
-
 Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.13 10
-
-
 Bacillus sp. (in: Bacteria)