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Literature summary extracted from

  • Nagai, Y.; Sugitani, T.; Tsuyuki, K.
    Characterization of alpha-glucosyltransferase from Pseudomonas mesoacidophila MX-45 (1994), Biosci. Biotechnol. Biochem., 58, 1789-1793.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.99.11 1,10-phenanthroline
-
 Paraburkholderia acidicola
5.4.99.11 Ag+
-
 Paraburkholderia acidicola
5.4.99.11 Fe3+
-
 Paraburkholderia acidicola
5.4.99.11 glucono-1,5-lactone
-
 Paraburkholderia acidicola
5.4.99.11 glucose
-
 Paraburkholderia acidicola
5.4.99.11 Hg2+
-
 Paraburkholderia acidicola
5.4.99.11 lactose
-
 Paraburkholderia acidicola
5.4.99.11 Mg2+
-
 Paraburkholderia acidicola
5.4.99.11 Pb2+
-
 Paraburkholderia acidicola
5.4.99.11 Tris(hydroxymethyl)aminomethane
-
 Paraburkholderia acidicola

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.11 0.0192
-
 sucrose
-
 Paraburkholderia acidicola

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.99.11 63000
-
 x * 63000, SDS-PAGE Paraburkholderia acidicola

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.11 Paraburkholderia acidicola
-
 MX-45
-
5.4.99.11 Paraburkholderia acidicola MX-45
-
 MX-45
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.11
-
 Paraburkholderia acidicola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.11 sucrose
-
 Paraburkholderia acidicola 6-O-alpha-D-Glucopyranosyl-D-fructofuranose isomaltulose + trehalulose. The ratio of trehalulose to isomaltulose increases at lower reaction temperatures. The optimal conditions for trehalulose production are pH 5.5-6.5 at 20 C ?
5.4.99.11 sucrose
-
 Paraburkholderia acidicola MX-45 6-O-alpha-D-Glucopyranosyl-D-fructofuranose isomaltulose + trehalulose. The ratio of trehalulose to isomaltulose increases at lower reaction temperatures. The optimal conditions for trehalulose production are pH 5.5-6.5 at 20 C ?

Subunits

EC Number Subunits Comment Organism
5.4.99.11 ? x * 63000, SDS-PAGE Paraburkholderia acidicola

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.99.11 additional information
-
 the ratio of trehalulose to isomaltulose increases at lower temperatures. The optimum for trehalulose production is 20°C Paraburkholderia acidicola
5.4.99.11 40
-
-
 Paraburkholderia acidicola

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.4.99.11 20 60 20°C: about 50% of maximal activity, 40°C: about 40% of maximal activity Paraburkholderia acidicola

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.4.99.11 40
-
 1 h, pH 5.6, stable up to Paraburkholderia acidicola
5.4.99.11 50
-
 1 h, pH 5.6, about 20% loss of activity Paraburkholderia acidicola
5.4.99.11 60
-
 1 h, pH 5.6, about 60% loss of activity Paraburkholderia acidicola

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.99.11 5.8
-
-
 Paraburkholderia acidicola

pH Range

EC Number pH Minimum pH Maximum Comment Organism
5.4.99.11 4.2 7.8 pH 4.2: about 30% of maximal activity, pH 7.8: about 50% of maximal activity Paraburkholderia acidicola

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.4.99.11 5.1 6.7 highest stability Paraburkholderia acidicola