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Literature summary extracted from

  • Nakajima, N.; Imaseki, H.
    Purification and properties of 1-aminocyclopropane-1-carboxylate synthase of mesocarp of Cucurbita maxima Duch. fruits (1986), Plant Cell Physiol., 27, 969-980.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.14 aminoethoxyvinylglycine
-
 Cucurbita maxima
4.4.1.14 aminooxyacetic acid
-
 Cucurbita maxima
4.4.1.14 Cu2+
-
 Cucurbita maxima
4.4.1.14 homocysteine slight Cucurbita maxima
4.4.1.14 S-adenosyl-L-methionine in presence of pyridoxal 5'-phosphate Cucurbita maxima
4.4.1.14 S-adenosylhomocysteine
-
 Cucurbita maxima
4.4.1.14 Semicarbazide
-
 Cucurbita maxima
4.4.1.14 Zn2+
-
 Cucurbita maxima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.14 0.0133
-
 S-adenosyl-L-methionine
-
 Cucurbita maxima
4.4.1.14 0.12
-
 S-adenosyl-L-methionine
-
 Cucurbita maxima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.4.1.14 additional information no effect: Mg2+, Mn2+, Co2+ Cucurbita maxima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.14 84000
-
 2 * 84000, SDS-PAGE Cucurbita maxima
4.4.1.14 160000
-
 gel filtration Cucurbita maxima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.14 S-adenosyl-L-methionine Cucurbita maxima biosynthesis of ethylene: plant hormone 1-aminocyclopropane-1-carboxylate + methylthioadenosine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.14 Cucurbita maxima
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.14
-
 Cucurbita maxima

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.4.1.14 fruit
-
 Cucurbita maxima
-
4.4.1.14 mesocarp
-
 Cucurbita maxima
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.14 additional information
-
-
 Cucurbita maxima
4.4.1.14 0.22
-
-
 Cucurbita maxima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.14 additional information not: S-adenosylhomocysteine Cucurbita maxima ?
-
 ?
4.4.1.14 additional information L-methionine Cucurbita maxima ?
-
 ?
4.4.1.14 additional information S-methylthionine Cucurbita maxima ?
-
 ?
4.4.1.14 S-adenosyl-L-methionine biosynthesis of ethylene: plant hormone Cucurbita maxima 1-aminocyclopropane-1-carboxylate + methylthioadenosine
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.14 dimer 2 * 84000, SDS-PAGE Cucurbita maxima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.14 9.5
-
-
 Cucurbita maxima

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.4.1.14 7 10
-
 Cucurbita maxima

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.14 pyridoxal 5'-phosphate small amounts, firmly bound Cucurbita maxima