Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dias, B.; Weimer, B.
    Purification and characterization of L-methionine gamma-lyase from Brevibacterium linens BL2 (1998), Appl. Environ. Microbiol., 64, 3327-3331.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.11 DL-propargylglycine
-
 Brevibacterium linens
4.4.1.11 hydroxylamine
-
 Brevibacterium linens
4.4.1.11 iodoacetate
-
 Brevibacterium linens
4.4.1.11 Penicillamine
-
 Brevibacterium linens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.11 6.12
-
 L-methionine
-
 Brevibacterium linens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.11 43000
-
 4 * 43000, SDS-PAGE Brevibacterium linens
4.4.1.11 170000
-
 gel filtration Brevibacterium linens

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.11 Brevibacterium linens
-
 BL2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.11
-
 Brevibacterium linens

Reaction

EC Number Reaction Comment Organism Reaction ID
4.4.1.11 L-methionine + H2O = methanethiol + NH3 + 2-oxobutanoate cleavage of C-N bond in addition to C-S, C-Se and C-O bond at beta-position of amino acids by elimination and replacement reactions Brevibacterium linens
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.11 ethionine
-
 Brevibacterium linens ?
-
 ?
4.4.1.11 L-methionine + H2O
-
 Brevibacterium linens methanethiol + NH3 + 2-oxobutanoate
-
 ?
4.4.1.11 S-methyl-L-cysteine + H2O
-
 Brevibacterium linens pyruvate + NH3 + CH3SH
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.11 tetramer 4 * 43000, SDS-PAGE Brevibacterium linens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.4.1.11 25
-
-
 Brevibacterium linens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.4.1.11 5 50
-
 Brevibacterium linens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.4.1.11 7.5 8
-
 Brevibacterium linens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.4.1.11 6 8
-
 Brevibacterium linens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.4.1.11 6 8 stable for 24 h Brevibacterium linens

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.11 pyridoxal 5'-phosphate
-
 Brevibacterium linens