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Literature summary extracted from
- Purification and characterization of cystathionine gamma-lyase from Lactobacillus fermentum DT41 (1998), FEMS Microbiol. Lett., 166, 197-202.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 4.4.1.1 | nutrition | Lactobacillus fermentum is contained in the natural starter used for producing Parmesan cheese and as adventitious non-starter lactic acid bacteria. It also populates several Italian and Swiss cheeses. EC 4.4.1.1 is stable in the conditions of cheese ripening and may contribute to the biosynthesis of sulfur-containing compounds | Limosilactobacillus fermentum |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.4.1.1 | 3-Methyl-2-benzothiazolinone hydrazone | - |
Limosilactobacillus fermentum |  |
| 4.4.1.1 | hydroxylamine | - |
Limosilactobacillus fermentum | |
| 4.4.1.1 | Semicarbazide | - |
Limosilactobacillus fermentum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.1 | 35000 | - |
4 * 35000, SDS-PAGE | Limosilactobacillus fermentum |
| 4.4.1.1 | 140000 | - |
non-denaturing PAGE, gel filtration | Limosilactobacillus fermentum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.4.1.1 | additional information | Limosilactobacillus fermentum | enzyme responsible for amino acid catabolism | ? | - |
? | |
| 4.4.1.1 | additional information | Limosilactobacillus fermentum DT41 | enzyme responsible for amino acid catabolism | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.4.1.1 | Limosilactobacillus fermentum | - |
DT41 | - |
| 4.4.1.1 | Limosilactobacillus fermentum DT41 | - |
DT41 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.4.1.1 | - |
Limosilactobacillus fermentum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.1 | additional information | - |
- |
Limosilactobacillus fermentum |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.4.1.1 | -20°C, stable for 90 days | Limosilactobacillus fermentum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.4.1.1 | djenkolic acid | L-djenkolic acid | Limosilactobacillus fermentum | ? | - |
? | |
| 4.4.1.1 | L-Cys | - |
Limosilactobacillus fermentum | H2S + NH3 + pyruvate | - |
? | |
| 4.4.1.1 | L-cystathionine + H2O | alpha,gamma-elimination | Limosilactobacillus fermentum | L-cysteine + 2-oxobutanoate + NH3 | - |
? | |
| 4.4.1.1 | L-cystathionine + H2O | alpha,gamma-elimination | Limosilactobacillus fermentum DT41 | L-cysteine + 2-oxobutanoate + NH3 | - |
? | |
| 4.4.1.1 | L-cystathionine + H2O | - |
Limosilactobacillus fermentum | L-homocysteine + pyruvate + NH3 | - |
? | |
| 4.4.1.1 | L-cystathionine + H2O | - |
Limosilactobacillus fermentum DT41 | L-homocysteine + pyruvate + NH3 | - |
? | |
| 4.4.1.1 | L-cysteine | best substrate | Limosilactobacillus fermentum | H2S + NH3 + pyruvate | - |
? | |
| 4.4.1.1 | L-cysteine | best substrate | Limosilactobacillus fermentum DT41 | H2S + NH3 + pyruvate | - |
? | |
| 4.4.1.1 | L-homocysteine | - |
Limosilactobacillus fermentum | ? | - |
? | |
| 4.4.1.1 | L-homoserine | - |
Limosilactobacillus fermentum | ? | - |
? | |
| 4.4.1.1 | L-Met | - |
Limosilactobacillus fermentum | methanethiol + ? | - |
? | |
| 4.4.1.1 | Lanthionine | - |
Limosilactobacillus fermentum | ? | - |
? | |
| 4.4.1.1 | Lanthionine | - |
Limosilactobacillus fermentum DT41 | ? | - |
? | |
| 4.4.1.1 | additional information | enzyme responsible for amino acid catabolism | Limosilactobacillus fermentum | ? | - |
? | |
| 4.4.1.1 | additional information | enzyme responsible for amino acid catabolism | Limosilactobacillus fermentum DT41 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.4.1.1 | tetramer | 4 * 35000, SDS-PAGE | Limosilactobacillus fermentum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.1 | 37 | - |
- |
Limosilactobacillus fermentum |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.1 | 10 | 40 | 10°C: about 35% of maximal activity, 40°C: rapid decrease of activity above | Limosilactobacillus fermentum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.1 | 20 | - |
20 h, stable | Limosilactobacillus fermentum |
| 4.4.1.1 | 65 | - |
1 min, complete inactivation | Limosilactobacillus fermentum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.1 | 8 | - |
- |
Limosilactobacillus fermentum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.1 | 5 | 8.5 | pH 5.0: about 60% of maximal activity, pH 8.5: about 55% of maximal activity | Limosilactobacillus fermentum |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.4.1.1 | pyridoxal 5'-phosphate | - |
Limosilactobacillus fermentum | |
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