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Literature summary extracted from
- Overexpression, purification, and some properties of the AdoCbl-dependent ethanolamine ammonia-lyase from Salmonella typhimurium (1992), Arch. Biochem. Biophys., 294, 50-54.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.3.1.7 | overexpression in Escherichia coli | Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.7 | 35000 | - |
alpha6beta6, 6 * 35000 + 6 * 50000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
| 4.3.1.7 | 50000 | - |
alpha6beta6, 6 * 35000 + 6 * 50000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
| 4.3.1.7 | 480000 | - |
gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.3.1.7 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.3.1.7 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.3.1.7 | 25.3 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.3.1.7 | ethanolamine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | acetaldehyde + NH3 | - |
? |  |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.3.1.7 | dodecamer | alpha6beta6, 6 * 35000 + 6 * 50000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.3.1.7 | adenosylcobalamin | dependent on | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 4.3.1.7 | adenosylcobalamin | Km 0.0005 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
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