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Literature summary extracted from

  • Hernandez, D.; Phillips, A.T.
    Purification and Characterization of Pseudomonas putida histidine ammonia-lyase expressed in Escherichia coli (1993), Protein Expr. Purif., 4, 473-478.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.1.3 expression in Escherichia coli Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.3 CN- KCN Pseudomonas putida
4.3.1.3 CN- KCN Pseudomonas sp.
4.3.1.3 D-histidine cloned enzyme, reversible competitive Pseudomonas putida
4.3.1.3 EDTA 0.005 mM, 75% decrease of activity Pseudomonas putida
4.3.1.3 EDTA
-
 Pseudomonas sp.
4.3.1.3 L-cysteine competitive, cloned enzyme Pseudomonas putida
4.3.1.3 L-cysteine
-
 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.3 5.3
-
 L-histidine native enzyme, pH 9.0 Pseudomonas putida
4.3.1.3 5.8
-
 L-histidine cloned enzyme, pH 9.0 Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.1.3 Mg2+
-
 Pseudomonas putida
4.3.1.3 Ni2+
-
 Pseudomonas putida
4.3.1.3 Zn2+
-
 Pseudomonas sp.
4.3.1.3 Zn2+
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.3 53700
-
 4 * 53700, calculation from sequence of amino acid Pseudomonas putida
4.3.1.3 220000
-
 gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.3 Pseudomonas putida
-
 ATCC 12633
-
4.3.1.3 Pseudomonas putida
-
 PRS1
-
4.3.1.3 Pseudomonas putida PRS1
-
 PRS1
-
4.3.1.3 Pseudomonas sp.
-
 ATCC 11,299b
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.3 additional information
-
-
 Pseudomonas sp.
4.3.1.3 additional information
-
-
 Pseudomonas putida
4.3.1.3 70.4
-
 cloned enzyme Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.3 L-histidine natural substrate Pseudomonas sp. urocanate + NH3
-
 ?
4.3.1.3 L-histidine natural substrate Pseudomonas putida urocanate + NH3
-
 ?
4.3.1.3 L-histidine non-oxidative deamination Pseudomonas putida urocanate + NH3
-
 ?
4.3.1.3 L-histidine natural substrate Pseudomonas putida PRS1 urocanate + NH3
-
 ?
4.3.1.3 L-histidine non-oxidative deamination Pseudomonas putida PRS1 urocanate + NH3
-
 ?
4.3.1.3 L-histidine methyl ester cloned enzyme Pseudomonas putida urocanate methyl ester cloned enzyme ?
4.3.1.3 L-histidine methyl ester cloned enzyme Pseudomonas putida PRS1 urocanate methyl ester cloned enzyme ?

Subunits

EC Number Subunits Comment Organism
4.3.1.3 tetramer
-
 Pseudomonas sp.
4.3.1.3 tetramer 4 * 53700, calculation from sequence of amino acid Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.1.3 255
-
 L-histidine
-
 Pseudomonas putida