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Literature summary extracted from

  • Khanna, R.; Chang, T.M.S.
    Characterization of L-histidine ammonia-lyase immobilized by microencapsulation in artificial cells: preparation, kinetics, stability, and in vitro depletion of histidine (1990), Int. J. Artif. Organs, 13, 189-195.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.3.1.3 synthesis enzyme can be successfully microcapsulated within cellulose nitrate artificial cells Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.3 20
-
 L-histidine enzyme in solution or microencapsulated Pseudomonas fluorescens

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.3 Pseudomonas fluorescens
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.3 additional information
-
-
 Pseudomonas fluorescens

Storage Stability

EC Number Storage Stability Organism
4.3.1.3 37°C, enzyme in solution: 50% loss of activity after 9.5 days, microencapsulated enzyme: 50% loss of activity after 15 days Pseudomonas fluorescens
4.3.1.3 4°C, enzyme in solution: 27% loss of activity after 21 days, and 60% loss of activity after 77 days, microencapsulated enzyme: 5% loss of activity after 21 days, and less than 50% loss of activity after 77 days Pseudomonas fluorescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.3 L-histidine natural substrate Pseudomonas fluorescens urocanate + NH3
-
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