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Literature summary extracted from

  • Shibatani, T.; Kakimoto, T.; Chibata, I.
    Crystalline L-histidine ammonia-lyase of Achromobacter liquidum. Crystallization and enzymic properties (1975), Eur. J. Biochem., 55, 263-269.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.1.3
-
 Achromobacter liquidum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.3 1,2,4-triazole-DL-alanine competitive Achromobacter liquidum
4.3.1.3 2-methyl-DL-histidine competitive Achromobacter liquidum
4.3.1.3 2-thiazolyl-DL-alanine competitive Achromobacter liquidum
4.3.1.3 3-methyl-L-histidine competitive Achromobacter liquidum
4.3.1.3 alpha-methyl-DL-histidine competitive Achromobacter liquidum
4.3.1.3 Ba2+ 1 mM Achromobacter liquidum
4.3.1.3 beta-imidazole lactic acid competitive Achromobacter liquidum
4.3.1.3 EDTA
-
 Pseudomonas putida
4.3.1.3 EDTA reversed by addition of divalent cations Pseudomonas sp.
4.3.1.3 EDTA reversed by addition of divalent cations Rattus norvegicus
4.3.1.3 Fe3+ 1 mM Achromobacter liquidum
4.3.1.3 formyl-L-histidine competitive Achromobacter liquidum
4.3.1.3 H2O2
-
 Achromobacter liquidum
4.3.1.3 hydroxymethylimidazole competitive Achromobacter liquidum
4.3.1.3 imidazole noncompetitive Achromobacter liquidum
4.3.1.3 L-cysteine
-
 Achromobacter liquidum
4.3.1.3 L-cysteine
-
 Pseudomonas putida
4.3.1.3 L-cysteine
-
 Pseudomonas sp.
4.3.1.3 L-histidine hydrazide noncompetitive Achromobacter liquidum
4.3.1.3 L-histidine hydroxamate competitive Achromobacter liquidum
4.3.1.3 L-histidine hydroxamate competitive Rattus norvegicus
4.3.1.3 L-histidinemethyl ester
-
 Achromobacter liquidum
4.3.1.3 L-histidinemethyl ester
-
 Rattus norvegicus
4.3.1.3 L-histidinol competitive Achromobacter liquidum
4.3.1.3 L-histidinol competitive Rattus norvegicus
4.3.1.3 NaBH4
-
 Achromobacter liquidum
4.3.1.3 NaBH4
-
 Pseudomonas sp.
4.3.1.3 NaBH4
-
 Rattus norvegicus
4.3.1.3 Pb2+ 1 mM Achromobacter liquidum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.3 3.6
-
 L-histidine
-
 Achromobacter liquidum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.3 200000
-
-
 Comamonas testosteroni
4.3.1.3 200000
-
 gel filtration Achromobacter liquidum

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.3 Achromobacter liquidum
-
 IAM 1667
-
4.3.1.3 Achromobacter liquidum IAM 1667
-
 IAM 1667
-
4.3.1.3 Bacillus cereus
-
-
-
4.3.1.3 Bacillus subtilis
-
-
-
4.3.1.3 Comamonas testosteroni
-
-
-
4.3.1.3 Helianthus sp.
-
 sunflower
-
4.3.1.3 Mycobacterium avium
-
-
-
4.3.1.3 Pseudomonas aeruginosa
-
-
-
4.3.1.3 Pseudomonas fluorescens
-
-
-
4.3.1.3 Pseudomonas putida
-
-
-
4.3.1.3 Pseudomonas sp.
-
 ATCC 11,299b
-
4.3.1.3 Rattus norvegicus
-
-
-
4.3.1.3 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
4.3.1.3 Spinacia oleracea
-
 spinach
-
4.3.1.3 Vibrio cholerae serotype O1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.3
-
 Achromobacter liquidum

Renatured (Commentary)

EC Number Renatured (Comment) Organism
4.3.1.3 crystalline enzyme is prepared with 24% recovery of activity Achromobacter liquidum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.3 63
-
-
 Achromobacter liquidum

Storage Stability

EC Number Storage Stability Organism
4.3.1.3 -20°C, 0.05 M potassium phosphate buffer, pH 7.0, no significant loss of activity for 2 years Pseudomonas sp.
4.3.1.3 5°C, crystalline enzyme in 3.5 M ammonium sulfate, stable for 1 year Achromobacter liquidum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.3 L-histidine natural substrate Salmonella enterica subsp. enterica serovar Typhimurium urocanate + NH3
-
 ?
4.3.1.3 L-histidine natural substrate Bacillus subtilis urocanate + NH3
-
 ?
4.3.1.3 L-histidine natural substrate Rattus norvegicus urocanate + NH3
-
 ?
4.3.1.3 L-histidine natural substrate Spinacia oleracea urocanate + NH3
-
 ?
4.3.1.3 L-histidine natural substrate Pseudomonas fluorescens urocanate + NH3
-
 ?
4.3.1.3 L-histidine natural substrate Pseudomonas aeruginosa urocanate + NH3
-
 ?
4.3.1.3 L-histidine natural substrate Pseudomonas sp. urocanate + NH3
-
 ?
4.3.1.3 L-histidine natural substrate Pseudomonas putida urocanate + NH3
-
 ?
4.3.1.3 L-histidine natural substrate Mycobacterium avium urocanate + NH3
-
 ?
4.3.1.3 L-histidine natural substrate Bacillus cereus urocanate + NH3
-
 ?
4.3.1.3 L-histidine natural substrate Comamonas testosteroni urocanate + NH3
-
 ?
4.3.1.3 L-histidine natural substrate Vibrio cholerae serotype O1 urocanate + NH3
-
 ?
4.3.1.3 L-histidine natural substrate Achromobacter liquidum urocanate + NH3
-
 ?
4.3.1.3 L-histidine natural substrate Helianthus sp. urocanate + NH3
-
 ?
4.3.1.3 L-histidine natural substrate Achromobacter liquidum IAM 1667 urocanate + NH3
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.1.3 50
-
-
 Achromobacter liquidum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.3.1.3 75
-
 15 min, stable for 30 min, without loss of activity Achromobacter liquidum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.3 8.2
-
-
 Achromobacter liquidum
4.3.1.3 8.8 9
-
 Rattus norvegicus
4.3.1.3 9
-
-
 Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.3.1.3 7.2 9.4 half-maximal activity at pH 7.2 and at pH 9.4 Achromobacter liquidum