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Literature summary extracted from

  • Rechler, M.M.; Tabor, H.
    Histidine ammonia-lyase (Pseudomonas) (1971), Methods Enzymol., 17B, 63-69.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.3.1.3 2-mercaptoethanol activates Pseudomonas sp.
4.3.1.3 dithiothreitol activates Pseudomonas sp.
4.3.1.3 GSH activates Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.3 biguanide
-
 Pseudomonas sp.
4.3.1.3 bisulfite
-
 Pseudomonas putida
4.3.1.3 bisulfite sodiumbisulfite Pseudomonas sp.
4.3.1.3 CN- KCN Pseudomonas putida
4.3.1.3 CN- KCN Pseudomonas sp.
4.3.1.3 EDTA
-
 Pseudomonas putida
4.3.1.3 EDTA
-
 Pseudomonas sp.
4.3.1.3 hydroxylamine
-
 Pseudomonas sp.
4.3.1.3 L-cysteine
-
 Pseudomonas putida
4.3.1.3 L-cysteine
-
 Pseudomonas sp.
4.3.1.3 L-tyrosine heat-purified enzyme, at low concentrations, pH 7-8 Pseudomonas sp.
4.3.1.3 NaBH4
-
 Pseudomonas sp.
4.3.1.3 p-hydroxyphenylpyruvate heat-purified enzyme, at low concentrations, pH 7-8 Pseudomonas sp.
4.3.1.3 p-mercuribenzoate inhibits mercaptoethanol-activated enzyme Pseudomonas sp.
4.3.1.3 phenylhydrazine
-
 Pseudomonas sp.
4.3.1.3 Sodium diphosphate
-
 Pseudomonas sp.
4.3.1.3 Urocanate
-
 Pseudomonas aeruginosa
4.3.1.3 Urocanate heat-purified enzyme, at low concentrations, pH 7-8 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.3 3.8
-
 L-histidine 25°C Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.3.1.3 Mn2+ enhances activity Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.1.3 210000
-
-
 Pseudomonas aeruginosa
4.3.1.3 210000
-
 sedimentation equilibrium Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.3 Pseudomonas aeruginosa
-
-
-
4.3.1.3 Pseudomonas putida
-
-
-
4.3.1.3 Pseudomonas sp.
-
 ATCC 11,299b
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.1.3
-
 Pseudomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.3 additional information
-
-
 Pseudomonas sp.
4.3.1.3 additional information
-
-
 Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
4.3.1.3 2°C, 0.1 M potassium phosphate, pH 7.2, 0.1 mM MnCl2 saturated with chloroform, stable for 9 months without loss of activity Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.3 L-histidine natural substrate Pseudomonas aeruginosa urocanate + NH3
-
 ?
4.3.1.3 L-histidine natural substrate Pseudomonas sp. urocanate + NH3
-
 ?
4.3.1.3 L-histidine natural substrate Pseudomonas putida urocanate + NH3
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.1.3 9
-
-
 Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.3.1.3 8.1 9.9 50% of maximal activity at pH 8.1 and pH 9.9 Pseudomonas sp.