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Literature summary extracted from
- Biosynthesis of DELTA-aminolevulinate in greening barley leaves: glutamate 1-semialdehyde aminotransferase (1978), Carlsberg Res. Commun., 43, 185-194.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.3.8 | Aminooxyacetate | 20 mM, 57% inhibition | Hordeum vulgare |  |
| 5.4.3.8 | cycloserine | 20 mM, 43% inhibition | Hordeum vulgare | |
| 5.4.3.8 | D-penicillamine | 20 mM, 9% inhibition | Hordeum vulgare | |
| 5.4.3.8 | glyoxylate | 20 mM, 27% inhibition | Hordeum vulgare | |
| 5.4.3.8 | additional information | no inhibition by 3-hydroxyglutamate | Hordeum vulgare | |
| 5.4.3.8 | p-chloromercuribenzoate | 0.1 mM, 45% inhibition | Hordeum vulgare | |
| 5.4.3.8 | pyridoxal 5'-phosphate | 5 mM, 47% inhibition | Hordeum vulgare | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.3.8 | 0.4 | - |
(S)-4-Amino-5-oxopentanoate | - |
Hordeum vulgare | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 5.4.3.8 | chloroplast | mature | Hordeum vulgare | 9507 | - |
| 5.4.3.8 | plastid | low specific activity in etiolated plastids compared to greening plastids | Hordeum vulgare | 9536 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.3.8 | 67000 | - |
x * 67000, SDS-PAGE | Hordeum vulgare |
| 5.4.3.8 | 500000 | - |
gel filtration | Hordeum vulgare |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.3.8 | (S)-4-Amino-5-oxopentanoate | Hordeum vulgare | the enzyme may catalyze a part reaction in the conversion of L-Glu to 5-amino-4-oxopentanoate in greening plastids | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.3.8 | Hordeum vulgare | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.3.8 | partial | Hordeum vulgare |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 5.4.3.8 | leaf | greening | Hordeum vulgare | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.3.8 | (S)-4-Amino-5-oxopentanoate | - |
Hordeum vulgare | 5-Amino-4-oxopentanoate | - |
? | |
| 5.4.3.8 | (S)-4-Amino-5-oxopentanoate | the enzyme may catalyze a part reaction in the conversion of L-Glu to 5-amino-4-oxopentanoate in greening plastids | Hordeum vulgare | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.3.8 | ? | x * 67000, SDS-PAGE | Hordeum vulgare |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.3.8 | 30 | - |
- |
Hordeum vulgare |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.3.8 | 14 | 30 | 14°C: 41% of maximal activity, 22°C: 76.4% of maximal activity | Hordeum vulgare |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.3.8 | 8 | - |
broad | Hordeum vulgare |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.3.8 | 6.7 | 8.8 | pH 6.7: about 45% of maximal activity, pH 8.8: about 90% of maximal activity | Hordeum vulgare |
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Release 2023.1 (January 2023)
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