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Literature summary extracted from

  • Nikaidou, N.; Naganuma, T.; Kamio, Y.; Izaki, K.
    Production, purification, and properties of a pectin lyase from Pseudomonas marginalis N6301 (1995), Biosci. Biotechnol. Biochem., 59, 323-324.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.10 malic anhydride 2 mM, complete inhibition Pseudomonas marginalis
4.2.2.10 N-Chlorosuccinimide 2 mM, 21% inhibition Pseudomonas marginalis
4.2.2.10 NEM 2 mM, 96% inhibition Pseudomonas marginalis
4.2.2.10 p-chloromercuriphenylsulfonic acid 2 mM, complete inhibition Pseudomonas marginalis
4.2.2.10 Phenylmethylsulfonylfluoride 2 mM, 9% inhibition Pseudomonas marginalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.10 34000
-
 x * 34000, SDS-PAGE Pseudomonas marginalis

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.10 Pseudomonas marginalis
-
 N-6031
-
4.2.2.10 Pseudomonas marginalis N-6031
-
 N-6031
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.10
-
 Pseudomonas marginalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.10 pectin
-
 Pseudomonas marginalis ?
-
 ?
4.2.2.10 pectin
-
 Pseudomonas marginalis N-6031 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.10 ? x * 34000, SDS-PAGE Pseudomonas marginalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.10 40
-
 pH 8.5, 30 min, stable below Pseudomonas marginalis
4.2.2.10 45
-
 rapid loss of activity Pseudomonas marginalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.10 8
-
-
 Pseudomonas marginalis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.10 5.2 8.1 stable Pseudomonas marginalis