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Literature summary extracted from

  • Wang, P.; Matthews, D.E.; VanEtten, H.D.
    Purification and characterization of cyanide hydratase from the phytopathogenic fungus Gloeocercospora sorghi (1992), Arch. Biochem. Biophys., 298, 569-575.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.66 Hg2+
-
 Microdochium sorghi
4.2.1.66 additional information not inhibited by Ag+, Zn2+, Cu2+, iodoacetamide Microdochium sorghi
4.2.1.66 p-chloromercuribenzoate
-
 Microdochium sorghi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.66 45000
-
 x * 45000, SDS-PAGE Microdochium sorghi
4.2.1.66 300000 1000000 gel filtration Microdochium sorghi

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.66 Aspergillus nidulans
-
-
-
4.2.1.66 Bipolaris sorghicola
-
-
-
4.2.1.66 Colletotrichum graminicola
-
-
-
4.2.1.66 Colletotrichum magnum
-
-
-
4.2.1.66 Curvularia lunata
-
-
-
4.2.1.66 Exserohilum turcicum
-
-
-
4.2.1.66 Fusarium verticillioides
-
-
-
4.2.1.66 Macrophomina phaseolina
-
-
-
4.2.1.66 Microdochium sorghi
-
-
-
4.2.1.66 Periconia circinata
-
-
-
4.2.1.66 Stemphylium vesicarium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.66
-
 Microdochium sorghi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.66 555
-
-
 Microdochium sorghi

Storage Stability

EC Number Storage Stability Organism
4.2.1.66 -80°C, more than 2 years Microdochium sorghi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.66 HCN + H2O
-
 Aspergillus nidulans formamide
-
 ?
4.2.1.66 HCN + H2O
-
 Fusarium verticillioides formamide
-
 ?
4.2.1.66 HCN + H2O
-
 Microdochium sorghi formamide
-
 ?
4.2.1.66 HCN + H2O
-
 Colletotrichum graminicola formamide
-
 ?
4.2.1.66 HCN + H2O
-
 Curvularia lunata formamide
-
 ?
4.2.1.66 HCN + H2O
-
 Bipolaris sorghicola formamide
-
 ?
4.2.1.66 HCN + H2O
-
 Macrophomina phaseolina formamide
-
 ?
4.2.1.66 HCN + H2O
-
 Periconia circinata formamide
-
 ?
4.2.1.66 HCN + H2O
-
 Colletotrichum magnum formamide
-
 ?
4.2.1.66 HCN + H2O
-
 Exserohilum turcicum formamide
-
 ?
4.2.1.66 HCN + H2O
-
 Stemphylium vesicarium formamide
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.66 polymer x * 45000, SDS-PAGE Microdochium sorghi