Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Smiley, K.L.; Sobolov, M.
    A cobamide-requiring glycerol dehydrase from an acrolein-forming Lactobacillus (1962), Arch. Biochem. Biophys., 97, 538-543.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.30 adenylcobamide activates Lactobacillus sp.
4.2.1.30 additional information
-
 Klebsiella pneumoniae
4.2.1.30 additional information
-
 Lactobacillus sp.
4.2.1.30 NH4+
-
 Klebsiella pneumoniae
4.2.1.30 NH4+ required, most active univalent cation Lactobacillus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.30 CN- KCN, pH 6.0, slowly inactivates Lactobacillus sp.
4.2.1.30 cyanocobalamin quite inhibitory to enzyme activity if it is added to the apoenzyme before the 5,6-dimethylbenzimidazolylcobamide Lactobacillus sp.
4.2.1.30 hydroxycobalamin
-
 Lactobacillus sp.
4.2.1.30 additional information
-
 Klebsiella pneumoniae
4.2.1.30 additional information
-
 Lactobacillus sp.
4.2.1.30 Na+
-
 Klebsiella pneumoniae
4.2.1.30 Na+
-
 Lactobacillus sp.
4.2.1.30 p-chloromercuribenzoate
-
 Klebsiella pneumoniae
4.2.1.30 p-chloromercuribenzoate 2,3-dimercaptopropanol, glutathione and cysteine protect Lactobacillus sp.
4.2.1.30 phenyl mercuri acetate
-
 Lactobacillus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.30 K+ required Klebsiella pneumoniae
4.2.1.30 K+ required Lactobacillus sp.
4.2.1.30 K+ 80-90% as active as NH4+ Lactobacillus sp.
4.2.1.30 Rb+ required, 80-90% as active as NH4+ Klebsiella pneumoniae
4.2.1.30 Rb+ required, 80-90% as active as NH4+ Lactobacillus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.30 glycerol Klebsiella pneumoniae
-
 ?
-
 ?
4.2.1.30 glycerol Lactobacillus sp.
-
 ?
-
 ?
4.2.1.30 glycerol Lactobacillus sp. 208-A
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.30 Klebsiella pneumoniae
-
-
-
4.2.1.30 Lactobacillus sp.
-
 208-A
-
4.2.1.30 Lactobacillus sp. 208-A
-
 208-A
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.30 additional information
-
-
 Klebsiella pneumoniae
4.2.1.30 additional information
-
-
 Lactobacillus sp.

Storage Stability

EC Number Storage Stability Organism
4.2.1.30 -20°C, soluble enzyme is stable for at least 2 weeks Lactobacillus sp.
4.2.1.30 4°C, soluble enzyme loses 25% of activity in 3 days Lactobacillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.30 glycerol
-
 Klebsiella pneumoniae 3-hydroxypropanal + H2O beta-hydroxypropionaldehyde ?
4.2.1.30 glycerol
-
 Lactobacillus sp. 3-hydroxypropanal + H2O beta-hydroxypropionaldehyde ?
4.2.1.30 glycerol
-
 Lactobacillus sp. 208-A 3-hydroxypropanal + H2O beta-hydroxypropionaldehyde ?
4.2.1.30 glycerol
-
 Klebsiella pneumoniae ?
-
 ?
4.2.1.30 glycerol
-
 Lactobacillus sp. ?
-
 ?
4.2.1.30 glycerol
-
 Lactobacillus sp. 208-A ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.30 5.8 6
-
 Lactobacillus sp.
4.2.1.30 8
-
-
 Klebsiella pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.30 5,6-Dimethylbenzimidazolylcobamide coenzyme required Klebsiella pneumoniae
4.2.1.30 5,6-Dimethylbenzimidazolylcobamide coenzyme required Lactobacillus sp.