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Literature summary extracted from
- Purification, characterization and preliminary X-ray study of fumarase from Saccharomyces cerevisiae (1992), Biochim. Biophys. Acta, 1122, 85-92.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.2 | acetate | 0.2 M, activation | Saccharomyces cerevisiae |  |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 4.2.1.2 | fumarase complex with meso-tartaric acid | Saccharomyces cerevisiae |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.2 | 50000 | - |
4 * 50000, SDS-PAGE | Saccharomyces cerevisiae |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.2 | Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.2 | - |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.2 | 1150 | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.2.1.2 | -4°C, stable for at least 6 months | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.2 | (S)-malate | - |
Saccharomyces cerevisiae | fumarate + H2O | - |
r | |
| 4.2.1.2 | fumarate + H2O | - |
Saccharomyces cerevisiae | L-malate | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.2 | tetramer | 4 * 50000, SDS-PAGE | Saccharomyces cerevisiae |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.2 | 50 | - |
complete inactivation within several seconds | Saccharomyces cerevisiae |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.2 | 4.5 | 8.2 | stable | Saccharomyces cerevisiae |
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Release 2023.1 (January 2023)
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