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Literature summary extracted from
- Physicochemical, catalytic, and immunochemical properties of fumarases crystallized separately from mitochondrial and cytosolic fractions of rat liver (1981), J. Biochem., 89, 1923-1931.
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 4.2.1.2 | - |
Rattus norvegicus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.2 | 0.013 | - |
fumarate | cytosolic enzyme | Rattus norvegicus |  |
| 4.2.1.2 | 0.14 | - |
(S)-malate | mitochondrial enzyme | Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.2.1.2 | cytosol | - |
Rattus norvegicus | 5829 | - |
| 4.2.1.2 | mitochondrion | cytosolic fumarase and mitochondrial fumarase are identical products of the same nuclear gene | Rattus norvegicus | 5739 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.2 | 49000 | - |
4 * 49000, SDS-PAGE in presence of 8 M urea | Rattus norvegicus |
| 4.2.1.2 | 195000 | - |
calculation from amino acid analysis | Rattus norvegicus |
| 4.2.1.2 | 200000 | - |
sucrose density gradient centrifugation | Rattus norvegicus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.2 | Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.2 | - |
Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.2.1.2 | liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.2 | 721 | - |
mitochondrial enzyme | Rattus norvegicus |
| 4.2.1.2 | 730 | - |
cytosolic enzyme | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.2 | (S)-malate | - |
Rattus norvegicus | fumarate + H2O | - |
r | |
| 4.2.1.2 | fumarate + H2O | - |
Rattus norvegicus | L-malate | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.2 | tetramer | 4 * 49000, SDS-PAGE in presence of 8 M urea | Rattus norvegicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.2 | 6.8 | 7.5 | - |
Rattus norvegicus |
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Release 2023.1 (January 2023)
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