Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Shibata, H.; Gardiner, W.E.; Schwartzbach, S.D.
    Purification, characterization, and immunological properties of fumarase from Euglena gracilis var. bacillaris (1985), J. Bacteriol., 164, 762-768.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.2 phosphate at 0.1 mM malate and 5 mM phosphate: 14% increase in activity. At 0.1 mM malate and 50 mM phosphate: 60% inhibition. At 1 mM malate and 10 mM phosphate: 80% stimulation Euglena gracilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.2 ATP 0.35 mM, 50% inhibition Euglena gracilis
4.2.1.2 phosphate at 0.1 mM malate and 5 mM: 14% increase in activity. At 0.1 mM malate and 50 mM phosphate: 60% inhibition. At 1 mM malate and 10 mM phosphate: 80% stimulation Euglena gracilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.2 0.031
-
 fumarate
-
 Euglena gracilis
4.2.1.2 1.4
-
 (S)-malate
-
 Euglena gracilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.2 60000
-
 2 * 60000, SDS-PAGE Euglena gracilis
4.2.1.2 120000
-
 gel filtration Euglena gracilis

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.2 Euglena gracilis
-
 var. bacillaris
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.2
-
 Euglena gracilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.2 1047
-
-
 Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.2 (S)-malate
-
 Euglena gracilis fumarate + H2O
-
 r
4.2.1.2 fumarate + H2O
-
 Euglena gracilis L-malate
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.1.2 dimer 2 * 60000, SDS-PAGE Euglena gracilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.2 4
-
 half-life: 4.6 h, at 0.0438 mg of protein per ml Euglena gracilis
4.2.1.2 20
-
 half-life: 1.6 h, at 0.0438 mg of protein per ml Euglena gracilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.2 8.4
-
-
 Euglena gracilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.2 0.35
-
 ATP
-
 Euglena gracilis