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Literature summary extracted from

  • Woods, S.A.; Schwartzbach, S.D.; Guest, J.R.
    Two biochemically distinct classes of fumarase in Escherichia coli (1988), Biochim. Biophys. Acta, 954, 14-26.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.2.1.2 enzyme form FUMB is extremely unstable Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.2 0.15
-
fumarate enzyme form FUMA Escherichia coli
4.2.1.2 0.39
-
fumarate enzyme form FUMC Escherichia coli
4.2.1.2 0.63
-
(S)-malate enzyme form FUMB Escherichia coli
4.2.1.2 1.1
-
(S)-malate enzyme form FUMA Escherichia coli
4.2.1.2 1.7
-
fumarate enzyme form FUMB Escherichia coli
4.2.1.2 2.94
-
L-malate enzyme form FUMC Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.2 50000
-
4 * 50000, enzyme form FUMC, SDS-PAGE Escherichia coli
4.2.1.2 61000
-
2 * 61000, enzyme form FUMA, SDS-PAGE Escherichia coli
4.2.1.2 120000
-
enzyme form FUMA, gel filtration Escherichia coli
4.2.1.2 200000
-
enzyme form FUMC, gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.2 Escherichia coli
-
3 fumarases FUMA, FUMB, and FUMC
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.2 (S)-malate
-
Escherichia coli fumarate + H2O
-
r
4.2.1.2 fumarate + H2O
-
Escherichia coli L-malate
-
r

Subunits

EC Number Subunits Comment Organism
4.2.1.2 dimer 2 * 61000, enzyme form FUMA, SDS-PAGE Escherichia coli
4.2.1.2 tetramer 4 * 50000, enzyme form FUMC, SDS-PAGE Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.2 50
-
80 min, 30% loss of activity of the enzyme form FUMC. Rapid inactivation of enzyme form FUMA and FUMB Escherichia coli