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Literature summary extracted from

  • Hill, R.L.; Teipel, J.W.
    Fumarase and crotonase (1971), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 5, 539-571.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.2 arsenate activates Sus scrofa
4.2.1.2 Borate activates Sus scrofa
4.2.1.2 citrate activates Sus scrofa
4.2.1.2 phosphate increases activity at less than 5 mM, competitive inhibition at higher concentrations Sus scrofa
4.2.1.2 selenate activates Sus scrofa
4.2.1.2 SO42- activates Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.2 4-bromocrotonate
-
 Sus scrofa
4.2.1.2 ATP
-
 Sus scrofa
4.2.1.2 bromofumarate
-
 Sus scrofa
4.2.1.2 chlorofumarate
-
 Sus scrofa
4.2.1.2 citrate
-
 Sus scrofa
4.2.1.2 CTP
-
 Sus scrofa
4.2.1.2 dimethylfumarate
-
 Sus scrofa
4.2.1.2 GTP
-
 Sus scrofa
4.2.1.2 iodoacetamide
-
 Sus scrofa
4.2.1.2 iodoacetate
-
 Sus scrofa
4.2.1.2 iodofumarate
-
 Sus scrofa
4.2.1.2 isocitrate L-isocitrate Sus scrofa
4.2.1.2 L-Tartrate
-
 Sus scrofa
4.2.1.2 L-threo-hydroxyaspartate
-
 Sus scrofa
4.2.1.2 mesaconate
-
 Sus scrofa
4.2.1.2 additional information substrate inhibition between 0.1 M and 1.0 M Sus scrofa
4.2.1.2 NEM
-
 Sus scrofa
4.2.1.2 phosphate increases activity at less than 5 mM, competitive inhibition at higher concentrations Sus scrofa
4.2.1.2 trans-aconitate
-
 Sus scrofa
4.2.1.2 UTP
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.2 0.005
-
 fumarate
-
 Sus scrofa
4.2.1.2 0.02 0.14 L-threo-chloromalate
-
 Sus scrofa
4.2.1.2 0.025
-
 L-malate
-
 Sus scrofa
4.2.1.2 0.027
-
 fluorofumarate
-
 Sus scrofa
4.2.1.2 0.11
-
 chlorofumarate
-
 Sus scrofa
4.2.1.2 0.11
-
 bromofumarate
-
 Sus scrofa
4.2.1.2 0.12
-
 iodofumarate
-
 Sus scrofa
4.2.1.2 0.145
-
 acetylene dicarboxylate
-
 Sus scrofa
4.2.1.2 0.51
-
 mesaconate
-
 Sus scrofa
4.2.1.2 1.3
-
 L-Tartrate
-
 Sus scrofa
4.2.1.2 7.5 10 L-threo-hydroxyaspartate
-
 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.2 48500
-
 4 * 48500, equilibrium sedimentation in 6 M guanidine HCl, pH 4.25 Sus scrofa
4.2.1.2 194000
-
 equilibrium sedimentation Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.2 Sus scrofa
-
 several isoenzymes
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.1.2 (S)-malate = fumarate + H2O mechanism Sus scrofa
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.2 heart
-
 Sus scrofa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.2 (S)-malate
-
 Sus scrofa fumarate + H2O
-
 r
4.2.1.2 acetylene dicarboxylate + H2O
-
 Sus scrofa oxaloacetate
-
 ?
4.2.1.2 alpha-fluorofumarate + H2O
-
 Sus scrofa oxaloacetate + ?
-
 ?
4.2.1.2 bromofumarate + H2O
-
 Sus scrofa ?
-
 ?
4.2.1.2 chlorofumarate + H2O
-
 Sus scrofa ?
-
 ?
4.2.1.2 fluorofumarate + H2O
-
 Sus scrofa ?
-
 ?
4.2.1.2 fumarate + H2O
-
 Sus scrofa L-malate
-
 r
4.2.1.2 iodofumarate + H2O
-
 Sus scrofa ?
-
 ?
4.2.1.2 L-tartrate
-
 Sus scrofa oxaloacetate + H2O
-
 ?
4.2.1.2 L-threo-chloro-L-malate
-
 Sus scrofa chlorofumarate + H2O
-
 ?
4.2.1.2 L-threo-hydroxyaspartate
-
 Sus scrofa ?
-
 ?
4.2.1.2 mesaconate + H2O
-
 Sus scrofa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.2 tetramer 4 * 48500, equilibrium sedimentation in 6 M guanidine HCl, pH 4.25 Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.2 additional information
-
 at low concentrations, phosphate, sulfate, borate, selenate, arsenate and citrate activate fumarase and shift somewhat the pH optimum to a more alkaline pH Sus scrofa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.1.2 5 9 stable in potassium phosphate buffer, above pH 10: dissociation into an inactive form Sus scrofa
4.2.1.2 6 10 dissociation below pH 6 and above pH 10, Tris-acetate buffer Sus scrofa