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Literature summary extracted from
- Enoyl-CoA hydratases from Clostridium acetobutylicum and Escherichia coli (1981), Methods Enzymol., 71, 421-430.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.150 | crotonyl-CoA | the enzyme is markedly sensitive to inhibition by crotonyl-CoA at concentrations greater than about 0.075 mM | Clostridium acetobutylicum |  |
| 4.2.1.150 | additional information | not inhibited by hexenoyl-CoA | Clostridium acetobutylicum |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.150 | 0.03 | - |
crotonyl-CoA | at pH 8.0 and 25°C | Clostridium acetobutylicum | |
| 4.2.1.150 | 0.13 | - |
hexenoyl-CoA | at pH 8.0 and 25°C | Clostridium acetobutylicum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.150 | 40000 | - |
4 * 40000, SDS-PAGE | Clostridium acetobutylicum |
| 4.2.1.150 | 158000 | - |
sedimentation equilibrium analysis and gel filtration | Clostridium acetobutylicum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.150 | crotonyl-CoA + H2O | Clostridium acetobutylicum | - |
3-hydroxybutyryl-CoA | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.150 | Clostridium acetobutylicum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.150 | acetone precipitation, Sephadex G-200 gel filtration, and DEAE-Sephadex column chromatography | Clostridium acetobutylicum |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.2.1.150 | 4 or -20°C, Tris-HCl, pH 8.0, rapid loss of activity | Clostridium acetobutylicum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.150 | crotonyl-CoA + H2O | - |
Clostridium acetobutylicum | 3-hydroxybutyryl-CoA | - |
? | |
| 4.2.1.150 | hexenoyl-CoA + H2O | - |
Clostridium acetobutylicum | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.150 | homotetramer | 4 * 40000, SDS-PAGE | Clostridium acetobutylicum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.1.150 | crotonase | - |
Clostridium acetobutylicum |
| 4.2.1.150 | L-3-hydroxyacyl-CoA hydro-lyase | - |
Clostridium acetobutylicum |
| 4.2.1.150 | More | cf. EC 4.2.1.17 | Clostridium acetobutylicum |
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