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Literature summary extracted from

  • Gill, S.R.; Fedorka-Cray, P.J.; Tweten, R.K.; Sleeper, B.P.
    Purification and properties of the carbonic anhydrase of Rhodospirillum (1984), Arch. Microbiol., 138, 113-118.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.1 acetazolamide
-
Rhodospirillum rubrum
4.2.1.1 CN-
-
Rhodospirillum rubrum
4.2.1.1 DTNB
-
Rhodospirillum rubrum
4.2.1.1 I- NaI Rhodospirillum rubrum
4.2.1.1 imidazole
-
Rhodospirillum rubrum
4.2.1.1 iodoacetate
-
Rhodospirillum rubrum
4.2.1.1 N3-
-
Rhodospirillum rubrum
4.2.1.1 o-Iodosobenzoate
-
Rhodospirillum rubrum
4.2.1.1 phenyl methyl sulfonyl fluoride
-
Rhodospirillum rubrum
4.2.1.1 pyridoxal 5'-phosphate
-
Rhodospirillum rubrum
4.2.1.1 Rose bengal
-
Rhodospirillum rubrum
4.2.1.1 sulfanilamide
-
Rhodospirillum rubrum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.1 80
-
CO2
-
Rhodospirillum rubrum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.1 cytoplasm
-
Rhodospirillum rubrum 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.1 Zinc required for activity and stability Rhodospirillum rubrum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.1 14000
-
2 * 14000, SDS-PAGE Rhodospirillum rubrum
4.2.1.1 27000
-
sucrose density gradient centrifugation Rhodospirillum rubrum
4.2.1.1 30000
-
gel filtration Rhodospirillum rubrum

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Rhodospirillum rubrum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.1
-
Rhodospirillum rubrum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.1 additional information
-
-
Rhodospirillum rubrum

Storage Stability

EC Number Storage Stability Organism
4.2.1.1 -70°C, in presence of Zn2+ and 50% glycerol, stable for 3 months Rhodospirillum rubrum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 CO2 + H2O
-
Rhodospirillum rubrum H2CO3
-
?

Subunits

EC Number Subunits Comment Organism
4.2.1.1 dimer 2 * 14000, SDS-PAGE Rhodospirillum rubrum
4.2.1.1 hexamer 6 * 27000, SDS-PAGE Rhodospirillum rubrum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.1 7.5
-
-
Rhodospirillum rubrum